Difference between revisions of "Part:BBa K3328023"
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__NOTOC__ | __NOTOC__ | ||
| + | <partinfo>BBa_K3328023 short</partinfo> | ||
| + | realize the boolean calculation of NIMPLY | ||
| + | ===Design=== | ||
| + | In the NIMPLY gate, a deactivating RNA (INPUT A) uses direct hybridization or strand displacement to abolish trigger RNA (input B) activity. So when only the correct trigger RNA is expressed, the switch can be turned on. | ||
| + | https://2020.igem.org/wiki/images/thumb/8/87/T--OUC-China--design_lunbo_nimply.jpg/799px-T--OUC-China--design_lunbo_nimply.jpg | ||
| + | ===Result=== | ||
| + | https://2020.igem.org/wiki/images/thumb/7/78/T--OUC-China--part_NIMPLY1.jpg/800px-T--OUC-China--part_NIMPLY1.jpg | ||
| + | The left figure shows that when INPUT A=0, INPUT B=1, the fluorescence of GFP is high. And the fluorescence intensity of GFP was low in the other three groups. This corresponds to the situation described in the truth table on the right. INPUT A=1 means that aTc (0.25 mg/mL) is added, INPUT B=1 means that HSL (0.1 mg/mL) is added. Error bar: SD (n=9). | ||
| + | <!-- Add more about the biology of this part here | ||
| + | ===Usage and Biology=== | ||
| + | <!-- --> | ||
| + | <span class='h3bb'>Sequence and Features</span> | ||
| + | <partinfo>BBa_K3328023 SequenceAndFeatures</partinfo> | ||
| − | + | <!-- Uncomment this to enable Functional Parameter display | |
| − | + | ===Functional Parameters=== | |
| − | + | <partinfo>BBa_K3328023 parameters</partinfo> | |
| − | <partinfo>BBa_K3328023 | + | <!-- --> |
| − | + | ||
| − | < | + | |
Latest revision as of 11:07, 27 October 2020
switch of NIMPLY gate(NIMPLY1)
realize the boolean calculation of NIMPLY
Design
In the NIMPLY gate, a deactivating RNA (INPUT A) uses direct hybridization or strand displacement to abolish trigger RNA (input B) activity. So when only the correct trigger RNA is expressed, the switch can be turned on.
Result
The left figure shows that when INPUT A=0, INPUT B=1, the fluorescence of GFP is high. And the fluorescence intensity of GFP was low in the other three groups. This corresponds to the situation described in the truth table on the right. INPUT A=1 means that aTc (0.25 mg/mL) is added, INPUT B=1 means that HSL (0.1 mg/mL) is added. Error bar: SD (n=9).
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 746