Difference between revisions of "Part:BBa K3611000"
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<partinfo>BBa_K3611000 short</partinfo> | <partinfo>BBa_K3611000 short</partinfo> | ||
| − | This part is the coding gene of the recombinant protein of human angiotensin-converting enzyme 2 (ACE2) and bacteria PmrB protein of PmrA/PmrB | + | |
| − | We replaced the original PmrB' s Fe (III) sensitive domains Trp34 to Glu64 with the core binding domain of ACE2 protein Thr20 to Asn90 in order to receive stimulation from the S protein of SARS-CoV-2. Upon sensing S protein of SARS-CoV-2, the sensor kinase PmrB autophosphorylates a highly conserved histidine residue and subsequently transfers the phosphoryl group to a conserved aspartate residue in its cognate response regulator PmrA. | + | This part is the coding gene of the recombinant protein of human angiotensin-converting enzyme 2 (ACE2) and bacteria PmrB protein of PmrA/PmrB two component system. |
| + | |||
| + | We replaced the original PmrB' s Fe (III) sensitive domains Trp34 to Glu64 with the core binding domain of ACE2 protein Thr20 to Asn90 in order to receive stimulation from the S protein of SARS-CoV-2. Upon sensing S protein of SARS-CoV-2, the sensor kinase PmrB autophosphorylates a highly conserved histidine residue and subsequently transfers the phosphoryl group to a conserved aspartate residue in its cognate response regulator PmrA. | ||
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<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> | ||
| − | <partinfo> | + | <partinfo>BBa_K3611000 SequenceAndFeatures</partinfo> |
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Latest revision as of 12:04, 20 October 2020
The coding gene of ACE2-PmrB recombinant protein.
This part is the coding gene of the recombinant protein of human angiotensin-converting enzyme 2 (ACE2) and bacteria PmrB protein of PmrA/PmrB two component system.
We replaced the original PmrB' s Fe (III) sensitive domains Trp34 to Glu64 with the core binding domain of ACE2 protein Thr20 to Asn90 in order to receive stimulation from the S protein of SARS-CoV-2. Upon sensing S protein of SARS-CoV-2, the sensor kinase PmrB autophosphorylates a highly conserved histidine residue and subsequently transfers the phosphoryl group to a conserved aspartate residue in its cognate response regulator PmrA.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 1156
- 1000COMPATIBLE WITH RFC[1000]