Difference between revisions of "Part:BBa K3468075"
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<partinfo>BBa_K3468075 SequenceAndFeatures</partinfo> | <partinfo>BBa_K3468075 SequenceAndFeatures</partinfo> | ||
| + | There is new salt bridge and hydrogen bond increased between A40D and K252. Salt bridge is a kind of electric interaction. | ||
| + | We use FoldX to judge the thermal stability of A40D and Jmol to find whether there is a new salt bridge. Finally we use Pymol to find out whether there is other interactions to make it become more stable. | ||
| + | [[File:A40D.png|400px|thumb|left|Fig.1 A40D in PyMOL]] | ||
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Latest revision as of 14:02, 27 October 2020
PETase A40D
The PETase is an enzyme, which can hydrolyze PET and this mutation protein is changed on the basis of the PETase. This protein is changed from A to D at 40 position which can be more stable in higher temperature compared with the wild type.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
There is new salt bridge and hydrogen bond increased between A40D and K252. Salt bridge is a kind of electric interaction. We use FoldX to judge the thermal stability of A40D and Jmol to find whether there is a new salt bridge. Finally we use Pymol to find out whether there is other interactions to make it become more stable.
