Difference between revisions of "Part:BBa K3329062"

 
 
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<partinfo>BBa_K3329062 short</partinfo>
 
<partinfo>BBa_K3329062 short</partinfo>
  
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SpyTag003 is a sub part in the Spytag-catcher003 system. It should be used with Spycatcher003. The cellulose binding module help connect cellulose and  Spycatcher003. The monomeric red fluorescent protein is connected with CBM. The super-folder GFP protein is connected with scl2. The green color indicates whether the scl2 is bound to cellulose by seeing whether that filter paper turned green.  
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SpyTag003 is a sub part in the Spytag-catcher003 system. It should be used with Spycatcher003. The super-folder GFP protein is connected with scl2. The green color indicates whether the scl2 is bound to cellulose by seeing whether that filter paper turned green.  
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 12:10, 20 October 2020


SpyTag003*3-Scl2*2-sfGFP for P. Pastoris

SpyTag003 is a sub part in the Spytag-catcher003 system. It should be used with Spycatcher003. The super-folder GFP protein is connected with scl2. The green color indicates whether the scl2 is bound to cellulose by seeing whether that filter paper turned green.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 560
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 787
    Illegal NgoMIV site found at 1116
    Illegal NgoMIV site found at 1663
    Illegal NgoMIV site found at 1695
    Illegal AgeI site found at 1492
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 862
    Illegal BsaI.rc site found at 1588