Difference between revisions of "Part:BBa K3468073"
| (One intermediate revision by one other user not shown) | |||
| Line 11: | Line 11: | ||
<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> | ||
<partinfo>BBa_K3468073 SequenceAndFeatures</partinfo> | <partinfo>BBa_K3468073 SequenceAndFeatures</partinfo> | ||
| + | Adding hydrogen bonds stabilized α1-β1loop. | ||
| + | It was predicted by FoldX that DDG decreased, and PyOML was used for visual screening, which did not form hydrogen bonds. after mutating it into ASP, it formed hydrogen bonds with SER-46, which stabilized α1-β1loop and was beneficial to improve the thermal stability of PETase. | ||
| + | [[File:Ala47.png|400px|thumb|left|Fig.1 Ala47 in PyMOL]] | ||
| + | [[File:A47D.png|400px|thumb|left|Fig.2 A47D in PyMOL]] | ||
<!-- Uncomment this to enable Functional Parameter display | <!-- Uncomment this to enable Functional Parameter display | ||
Latest revision as of 14:00, 27 October 2020
PETase A47D
The PETase is an enzyme, which can hydrolyze PET and this mutation protein is changed on the basis of the PETase. This protein is changed from A to R at 47 position which can be more stable in higher temperature compared with the wild type.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Adding hydrogen bonds stabilized α1-β1loop.
It was predicted by FoldX that DDG decreased, and PyOML was used for visual screening, which did not form hydrogen bonds. after mutating it into ASP, it formed hydrogen bonds with SER-46, which stabilized α1-β1loop and was beneficial to improve the thermal stability of PETase.
