Difference between revisions of "Part:BBa K3611000"

 
 
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<partinfo>BBa_K3611000 short</partinfo>
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This part is the coding gene of the recombinant protein of human angiotensin-converting enzyme 2 (ACE2) and bacteria PmrB protein of PmrA/PmrB two component system.
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We replaced the original PmrB' s Fe (III) sensitive domains Trp34 to Glu64 with the core binding domain of ACE2 protein Thr20 to Asn90 in order to receive stimulation from the S protein of SARS-CoV-2. Upon sensing S protein of SARS-CoV-2, the sensor kinase PmrB autophosphorylates a highly conserved histidine residue and subsequently transfers the phosphoryl group to a conserved aspartate residue in its cognate response regulator PmrA.
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===Usage and Biology===
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<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K3611000 SequenceAndFeatures</partinfo>
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===Functional Parameters===
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<partinfo>BBa_K3611000 parameters</partinfo>
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Latest revision as of 12:04, 20 October 2020


The coding gene of ACE2-PmrB recombinant protein.


This part is the coding gene of the recombinant protein of human angiotensin-converting enzyme 2 (ACE2) and bacteria PmrB protein of PmrA/PmrB two component system.

We replaced the original PmrB' s Fe (III) sensitive domains Trp34 to Glu64 with the core binding domain of ACE2 protein Thr20 to Asn90 in order to receive stimulation from the S protein of SARS-CoV-2. Upon sensing S protein of SARS-CoV-2, the sensor kinase PmrB autophosphorylates a highly conserved histidine residue and subsequently transfers the phosphoryl group to a conserved aspartate residue in its cognate response regulator PmrA.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1156
  • 1000
    COMPATIBLE WITH RFC[1000]