Difference between revisions of "Part:BBa K3468071"

 
 
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K3468071 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K3468071 SequenceAndFeatures</partinfo>
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Add hydrogen bonds.
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According to the prediction of FoldX, DDG decreased, and PyOML was used for visual screening, which did not form hydrogen bonds. after mutating it into SER, it formed hydrogen bonds with GLY-48 and SER-103, which stabilized α1-β1loop and was beneficial to improve the thermal stability of PETase.
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[[File:Ala47.png|400px|thumb|left|Fig.1 Ala47 in PyMOL]]
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[[File:A47S.png|400px|thumb|left|Fig.2 A47S in PyMOL]]
  
 
<!-- Uncomment this to enable Functional Parameter display  
 
<!-- Uncomment this to enable Functional Parameter display  

Latest revision as of 13:58, 27 October 2020


PETase A47S

The PETase is an enzyme, which can hydrolyze PET and this mutation protein is changed on the basis of the PETase. This protein is changed from A to S at 47 position which can be more stable in higher temperature compared with the wild type.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Add hydrogen bonds. According to the prediction of FoldX, DDG decreased, and PyOML was used for visual screening, which did not form hydrogen bonds. after mutating it into SER, it formed hydrogen bonds with GLY-48 and SER-103, which stabilized α1-β1loop and was beneficial to improve the thermal stability of PETase.

Fig.1 Ala47 in PyMOL
Fig.2 A47S in PyMOL