Difference between revisions of "Part:BBa K3402028"
 
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<partinfo>BBa_K3402028 short</partinfo>
 
<partinfo>BBa_K3402028 short</partinfo>
  
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This part consists of three short genes. <i>RS</i> and <i>HH</i>. are linked at the 3’ end of sg<i>GFP</i>([https://parts.igem.org/Part:BBa_K3402024# BBa_K3402024]), <i>HDV</i> is linked at the 5’ end of sg<i>GFP</i>.
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<i>HDV</i> is linked by the sgRNA.
  
 
===Usage and Biology===
 
===Usage and Biology===
  
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In the device that we want to express Cas9 protein, these three parts of genes will help in the formation of mature sgRNA.
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In the device that we want to express Cas9 gene, this gene will help in the formation of mature sgRNA.
  
 
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Latest revision as of 06:23, 21 October 2020


HDV

HDV is linked by the sgRNA.

Usage and Biology

In the device that we want to express Cas9 gene, this gene will help in the formation of mature sgRNA.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 33
    Illegal NgoMIV site found at 62
  • 1000
    COMPATIBLE WITH RFC[1000]