Difference between revisions of "Part:BBa K3468018:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | + | By observing and comparing the structure of PETase, appropriate mutations were constructed and the forces between the residues were modified. By searching for suitable sites around P276 and F284 for mutations, the hydrophobic interaction force between the residues was enhanced and the hydrophobic core was created, so N275I was designed. | |
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===Source=== | ===Source=== | ||
Latest revision as of 13:12, 23 October 2020
PETase N275I
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
By observing and comparing the structure of PETase, appropriate mutations were constructed and the forces between the residues were modified. By searching for suitable sites around P276 and F284 for mutations, the hydrophobic interaction force between the residues was enhanced and the hydrophobic core was created, so N275I was designed.
Source
Ideonalla sakaiensis