Difference between revisions of "Part:BBa K3425001:Design"
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===References=== | ===References=== |
Latest revision as of 20:07, 19 October 2020
pSB3C11: Improved pEven1 Loop Vector based on pSB3C01
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 2713
Illegal PstI site found at 12 - 12INCOMPATIBLE WITH RFC[12]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 2713
Illegal NheI site found at 1399
Illegal PstI site found at 12 - 21INCOMPATIBLE WITH RFC[21]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 2713 - 23INCOMPATIBLE WITH RFC[23]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 2713
Illegal PstI site found at 12 - 25INCOMPATIBLE WITH RFC[25]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 2713
Illegal PstI site found at 12
Illegal AgeI site found at 990
Illegal AgeI site found at 1313 - 1000INCOMPATIBLE WITH RFC[1000]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal BsaI site found at 2719
Illegal BsaI.rc site found at 6
Design Notes
A PCR mutagenesis was designed and conducted on pSB3C01 to remove the illegal BsaI restriction site. The primers used for this mutagenesis are Mut_L2_Fw and Mut_L2_Rev and they can be found in Team Uppsala 2020's Parts page.
Source
PCR mutagenesis from pSB3C01.