Difference between revisions of "Part:BBa K146000"

 
 
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__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K146000 short</partinfo>
 
<partinfo>BBa_K146000 short</partinfo>
  
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pCR2.1 was purchased from InVitrogen for clone part BBa_I764000 as a PCR product
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pCR2.1 was purchased from InVitrogen for cloning part BBa_I764001 as a PCR product.  Part BBa_I764001 was sequenced in this cloning vector using the M13 forward and M13 reverse sequencing primers.
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 14:46, 29 October 2008

pCR2.1 PCR product cloning vector

pCR2.1 was purchased from InVitrogen for cloning part BBa_I764001 as a PCR product. Part BBa_I764001 was sequenced in this cloning vector using the M13 forward and M13 reverse sequencing primers.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 3951
    Illegal suffix found in sequence at 1
    Illegal EcoRI site found at 28
    Illegal EcoRI site found at 3939
    Illegal XbaI site found at 73
    Illegal SpeI site found at 3914
    Illegal PstI site found at 33
    Illegal PstI site found at 1223
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 28
    Illegal EcoRI site found at 3939
    Illegal EcoRI site found at 3951
    Illegal SpeI site found at 2
    Illegal SpeI site found at 3914
    Illegal PstI site found at 16
    Illegal PstI site found at 33
    Illegal PstI site found at 1223
    Illegal NotI site found at 9
    Illegal NotI site found at 54
    Illegal NotI site found at 3957
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 28
    Illegal EcoRI site found at 3939
    Illegal EcoRI site found at 3951
    Illegal BglII site found at 1010
    Illegal BamHI site found at 3908
    Illegal XhoI site found at 61
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 3951
    Illegal suffix found in sequence at 2
    Illegal EcoRI site found at 28
    Illegal EcoRI site found at 3939
    Illegal XbaI site found at 73
    Illegal SpeI site found at 3914
    Illegal PstI site found at 33
    Illegal PstI site found at 1223
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 3951
    Illegal EcoRI site found at 28
    Illegal EcoRI site found at 3939
    Illegal XbaI site found at 73
    Illegal XbaI site found at 3966
    Illegal SpeI site found at 2
    Illegal SpeI site found at 3914
    Illegal PstI site found at 16
    Illegal PstI site found at 33
    Illegal PstI site found at 1223
    Illegal NgoMIV site found at 399
    Illegal NgoMIV site found at 1674
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2573
    Illegal SapI.rc site found at 1523
    Illegal SapI.rc site found at 1733
    Illegal SapI.rc site found at 3655