Difference between revisions of "Part:BBa K3468015:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | + | By observing and comparing the structure of PETase, appropriate mutations were constructed and the forces between the residues were modified. By finding suitable sites around A183, F201, and A240 for mutations, the hydrophobic interaction force between the residues is enhanced and the hydrophobic core is created, so Q182L was designed. | |
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===Source=== | ===Source=== | ||
Latest revision as of 11:41, 23 October 2020
PETase Q182L
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
By observing and comparing the structure of PETase, appropriate mutations were constructed and the forces between the residues were modified. By finding suitable sites around A183, F201, and A240 for mutations, the hydrophobic interaction force between the residues is enhanced and the hydrophobic core is created, so Q182L was designed.
Source
Ideonalla sakaiensis