Difference between revisions of "Part:BBa K3502000:Design"

 
 
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===Source===
 
===Source===
  
−
Jacob L. Litke 1,2 and Samie R. Jaffrey, Highly efficient expression of circular RNA aptamers in cells using autocatalytic transcripts, Nature biotechnology, 2018
+
 
  
 
===References===
 
===References===
 +
Jacob L. Litke 1,2 and Samie R. Jaffrey, Highly efficient expression of circular RNA aptamers in cells using autocatalytic transcripts, Nature biotechnology, 2018

Latest revision as of 18:22, 25 October 2020


The ribozyme sequence and ligation sequence added at both ends of dsRNA facilitate cyclization after


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 78
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 78
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 159
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 78
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 78
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

If you want to use this system in non-mammalian cells such as yeast cells, you may need to adjust the sequence of both ends according to the endogenous enzymes


Source

References

Jacob L. Litke 1,2 and Samie R. Jaffrey, Highly efficient expression of circular RNA aptamers in cells using autocatalytic transcripts, Nature biotechnology, 2018