Difference between revisions of "Part:BBa K3470004:Design"

 
(Design Notes)
 
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===Design Notes===
 
===Design Notes===
Gibson Assembly for assembly of parts
 
 
 
 
===Source===
 
 
E.coli DH5alpha
 
 
===References===
 

Latest revision as of 15:15, 23 October 2020


Modified mer Operon


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 652
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1878
    Illegal NgoMIV site found at 3162
    Illegal NgoMIV site found at 3210
    Illegal NgoMIV site found at 3272
    Illegal NgoMIV site found at 3483
    Illegal NgoMIV site found at 4088
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 4982
    Illegal SapI site found at 637


Design Notes