Difference between revisions of "Part:BBa K3264022:Experience"

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how you used this part and how it worked out.
 
how you used this part and how it worked out.
  
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===Applications of BBa_K3264022===
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== Pathway for BBa_K1131000==
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[[File:SZU2020 pET-28a(+)-CL34 Map.jpeg|320px]]
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Flaving Containing-Monooxygenase (FMO) is an enzyme that can be used to produce indigo. The enzyme is involved in the conversion of indole to indoxyl using NADPH as its co-factor. And upon exposure to oxygen, indoxyl spontaneously dimerzes to indigo. Specifically, this is the pathway being employed: <br> <br>
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try try
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[[File:HBHB.png|cebter|200px]]
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<!--Upon cloning this enzyme into E. coli with the [https://parts.igem.org/Part:BBa_J23100 BBa_J23100 Anderson Promoter], and adding tryptophan (a precursor to indole) in the LB media, we observed a dense blue color in our cultures. <br>[[File:blue tube.jpg | 350px]]-->
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===User Reviews===
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<!-- DON'T DELETE --><partinfo>BBa_K3264022 StartReviews</partinfo>
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{|width='80%' style='border:1px solid gray'
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<partinfo>BBa_K3264022 AddReview number</partinfo>
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<I>SZU-China</I>
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test test
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<!-- DON'T DELETE --><partinfo>BBa_K3264022 EndReviews</partinfo>
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Latest revision as of 15:26, 16 October 2020


This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Pathway for BBa_K1131000

Flaving Containing-Monooxygenase (FMO) is an enzyme that can be used to produce indigo. The enzyme is involved in the conversion of indole to indoxyl using NADPH as its co-factor. And upon exposure to oxygen, indoxyl spontaneously dimerzes to indigo. Specifically, this is the pathway being employed:

cebter