Difference between revisions of "Part:BBa K3264022:Experience"
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− | == | + | == Pathway for BBa_K1131000== |
− | + | Flaving Containing-Monooxygenase (FMO) is an enzyme that can be used to produce indigo. The enzyme is involved in the conversion of indole to indoxyl using NADPH as its co-factor. And upon exposure to oxygen, indoxyl spontaneously dimerzes to indigo. Specifically, this is the pathway being employed: <br> <br> | |
− | [[File: | + | [[File:HBHB.png|cebter|200px]] |
− | + | <!--Upon cloning this enzyme into E. coli with the [https://parts.igem.org/Part:BBa_J23100 BBa_J23100 Anderson Promoter], and adding tryptophan (a precursor to indole) in the LB media, we observed a dense blue color in our cultures. <br>[[File:blue tube.jpg | 350px]]--> | |
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Latest revision as of 15:26, 16 October 2020
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how you used this part and how it worked out.
Pathway for BBa_K1131000
Flaving Containing-Monooxygenase (FMO) is an enzyme that can be used to produce indigo. The enzyme is involved in the conversion of indole to indoxyl using NADPH as its co-factor. And upon exposure to oxygen, indoxyl spontaneously dimerzes to indigo. Specifically, this is the pathway being employed: