Difference between revisions of "Part:BBa K3610043:Design"
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===Design Notes=== | ===Design Notes=== | ||
− | + | HA tag fused to the luciferase for Western Blotting | |
===Source=== | ===Source=== | ||
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===References=== | ===References=== | ||
+ | Kunze, Gernot; Zipfel, Cyril; Robatzek, Silke; Niehaus, Karsten; Boller, Thomas; Felix, Georg (2004): The N terminus of bacterial elongation factor Tu elicits innate immunity in Arabidopsis plants. In: Plant Cell 16 (12), S. 3496–3507. DOI: 10.1105/tpc.104.026765. | ||
+ | |||
+ | England, Christopher G.; Ehlerding, Emily B.; Cai, Weibo (2016): NanoLuc: A Small Luciferase Is Brightening Up the Field of Bioluminescence. In: Bioconjug Chem 27 (5), S. 1175–1187. DOI: 10.1021/acs.bioconjchem.6b00112. |
Latest revision as of 22:11, 16 October 2020
EFR ectodomain / SmallBit for S. cerevisiae
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 355
Illegal NheI site found at 1285
Illegal NheI site found at 2203 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1871
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
HA tag fused to the luciferase for Western Blotting
Source
BBa_K319005, BBa_K2809002, BBa_K3610007, BBa_K416001, BBa_K3610016, BBa_K2753050
References
Kunze, Gernot; Zipfel, Cyril; Robatzek, Silke; Niehaus, Karsten; Boller, Thomas; Felix, Georg (2004): The N terminus of bacterial elongation factor Tu elicits innate immunity in Arabidopsis plants. In: Plant Cell 16 (12), S. 3496–3507. DOI: 10.1105/tpc.104.026765.
England, Christopher G.; Ehlerding, Emily B.; Cai, Weibo (2016): NanoLuc: A Small Luciferase Is Brightening Up the Field of Bioluminescence. In: Bioconjug Chem 27 (5), S. 1175–1187. DOI: 10.1021/acs.bioconjchem.6b00112.