Difference between revisions of "Part:BBa K3610039:Design"

(Design Notes)
(Design Notes)
 
Line 7: Line 7:
  
 
===Design Notes===
 
===Design Notes===
None
+
HA tag added between linker and mCherry for Western Blotting
  
 
===Source===
 
===Source===

Latest revision as of 10:59, 20 October 2020


EFR ectodomain / mCherry N-terminal for S. cerevisiae


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 355
    Illegal NheI site found at 1285
    Illegal NheI site found at 2203
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1871
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

HA tag added between linker and mCherry for Western Blotting

Source

BBa_K319005, BBa_K2809002, BBa_K3610007, BBa_K416001, BBa_K1150016, BBa_K3610020, BBa_K2753050

References

Fan, Jin-Yu; Cui, Zong-Qiang; Wei, Hong-Ping; Zhang, Zhi-Ping; Zhou, Ya-Feng; Wang, Yun-Peng; Zhang, Xian-En (2008): Split mCherry as a new red bimolecular fluorescence complementation system for visualizing protein–protein interactions in living cells. In: Biochemical and Biophysical Research Communications 367 (1), S. 47–53. DOI: 10.1016/j.bbrc.2007.12.101.

Kunze, Gernot; Zipfel, Cyril; Robatzek, Silke; Niehaus, Karsten; Boller, Thomas; Felix, Georg (2004): The N terminus of bacterial elongation factor Tu elicits innate immunity in Arabidopsis plants. In: Plant Cell 16 (12), S. 3496–3507. DOI: 10.1105/tpc.104.026765.