Difference between revisions of "Part:BBa K3610009"
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===Usage and Biology=== | ===Usage and Biology=== | ||
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| + | [[File:T--UZurich--FLS2 ecto.png|300px|thumb|left|Figure 1: FLS2 (Uniprot Q9FL28)]] | ||
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The FLAGELLIN-SENSITIVE 2 (FLS2) receptor is a plant pattern recognition receptor (PRR) and perceives bacterial flagellin, more specifically the epitope flg22. FLS2 interacts with another cell surface receptor BAK1. The heterodimerization of FLS2 and BAK1 induces an antibacterial immune response in the plant. | The FLAGELLIN-SENSITIVE 2 (FLS2) receptor is a plant pattern recognition receptor (PRR) and perceives bacterial flagellin, more specifically the epitope flg22. FLS2 interacts with another cell surface receptor BAK1. The heterodimerization of FLS2 and BAK1 induces an antibacterial immune response in the plant. | ||
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The ectodomain of the receptor can be used for protein-fragment complementation experiments (PCA). It can be fused to a split protein and coexpressed with BAK1, fused to the counterpart of the split protein, to visualize the interaction between the two receptors. | The ectodomain of the receptor can be used for protein-fragment complementation experiments (PCA). It can be fused to a split protein and coexpressed with BAK1, fused to the counterpart of the split protein, to visualize the interaction between the two receptors. | ||
Examples of such a complementation system are split-mCherry and the Split-NanoLuc system. | Examples of such a complementation system are split-mCherry and the Split-NanoLuc system. | ||
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Latest revision as of 21:23, 27 October 2020
FLS2 ectodomain from S. lycopersicum
This part is the ectodomain of the FLS2 receptor from S. lycopersicum.
Usage and Biology
The FLAGELLIN-SENSITIVE 2 (FLS2) receptor is a plant pattern recognition receptor (PRR) and perceives bacterial flagellin, more specifically the epitope flg22. FLS2 interacts with another cell surface receptor BAK1. The heterodimerization of FLS2 and BAK1 induces an antibacterial immune response in the plant.
FLS2 is a cell surface receptor consisting of an extracellular domain to which the ligand binds, a single-pass transmembrane domain and an cytoplasmatic kinase domain. Important for the interactoin with BAK1 are the transmembrane domain and the extracellular domain.
The ectodomain of the receptor can be used for protein-fragment complementation experiments (PCA). It can be fused to a split protein and coexpressed with BAK1, fused to the counterpart of the split protein, to visualize the interaction between the two receptors.
Examples of such a complementation system are split-mCherry and the Split-NanoLuc system.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 847
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 2086
Illegal AgeI site found at 472
Illegal AgeI site found at 760 - 1000COMPATIBLE WITH RFC[1000]