Difference between revisions of "Part:BBa K103006:Design"

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===Design Notes===
 
===Design Notes===
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[http://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=5&arg0=18_September_2008&arg1=29_September_2008&arg2=30_September_2008&arg3=2_October_2008&arg4=3_October_2008&name=Preparation%20of%20OmpA-linker%20%28BBa_K103006%29 Preparation of OmpA-linker (BBa_K103006)] (Univeristy of Warsaw 2008 iGEM team notebook)
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Preparation of BBa_K103003 is in details described [http://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=5&arg0=18_September_2008&arg1=29_September_2008&arg2=30_September_2008&arg3=2_October_2008&arg4=3_October_2008&name=Preparation%20of%20OmpA-linker%20%28BBa_K103006%29 here] (entries from Univeristy of Warsaw 2008 iGEM team notebook).
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OmpA_linker fragment was cutted from [http://2008.igem.org/Wiki/Team:Warsaw/vectors/pET15b%2BOmpA-alpha pET15b+OmpA-alpha] with SacI and NdeI. Resulting fragment was then ligated into [https://parts.igem.org/wiki/index.php?title=Part:pSB1A3 pSB1A3] ([https://parts.igem.org/wiki/index.php?title=Part:pSB1A3 pSB1A3] with NdeI and SacI sites was obtained by SacI/NdeI digest of [https://parts.igem.org/wiki/index.php?title=Part:pSB1A3 pSB1A3] carrying [https://parts.igem.org/wiki/index.php?title=Part:BBa_K103003 BBa_K103003])
  
 
===Source===
 
===Source===

Latest revision as of 13:56, 28 October 2008

OmpA outer membrane protein A fused to linker; displays proteins on cell surface


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Preparation of BBa_K103003 is in details described [http://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=5&arg0=18_September_2008&arg1=29_September_2008&arg2=30_September_2008&arg3=2_October_2008&arg4=3_October_2008&name=Preparation%20of%20OmpA-linker%20%28BBa_K103006%29 here] (entries from Univeristy of Warsaw 2008 iGEM team notebook).

OmpA_linker fragment was cutted from [http://2008.igem.org/Wiki/Team:Warsaw/vectors/pET15b%2BOmpA-alpha pET15b+OmpA-alpha] with SacI and NdeI. Resulting fragment was then ligated into pSB1A3 (pSB1A3 with NdeI and SacI sites was obtained by SacI/NdeI digest of pSB1A3 carrying BBa_K103003)

Source

References