Difference between revisions of "Part:BBa K115017"
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<partinfo>BBa_K115017 parameters</partinfo> | <partinfo>BBa_K115017 parameters</partinfo> | ||
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+ | <!--Rice iGEM 2019 improvement --> | ||
+ | ===Characterization=== | ||
+ | <html> | ||
+ | <p>To characterize this RNA thermometer in vivo, we made an RNA thermometer testing circuit, transformed it into <i>E. coli</i>, and tested the fluorescent output at different temperatures. These graphs show the relative fluorescent intensity of each thermometer between either 25 °C and 30 °C or 25 °C and 37 °C</p> | ||
+ | <img src="https://2019.igem.org/wiki/images/a/a2/T--Rice--RNATcassette.svg" width = 60%> | ||
+ | <p><b>Figure 1.</b>RNA thermometer testing circuit</p> | ||
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+ | <img src="https://2019.igem.org/wiki/images/1/16/T--Rice--BBa115017.png" width = 60%> | ||
+ | <p><b>Figure 2.</b> Fluorescence intensity at 25 °C, 30 °C, and 37 °C.</p> | ||
+ | </html> |
Latest revision as of 00:30, 22 October 2019
RNA thermometer (ROSE 32C)
An [http://2008.igem.org/Team:TUDelft/Temperature_analysis#RNA_thermometer RNA thermometer] which can be used for temperature sensitive post-transcriptional regulation, and which is designed to initiate translation around 32°C.
The image shows the secondary structure of the part after ligation to a protein coding part, as predicted by [http://rna.tbi.univie.ac.at/cgi-bin/RNAfold.cgi RNAfold]. Notice that the 3' end, including the scar and the start codon, does not belong to the part. The blue region is the Shine Dalgarno sequence which is the ribosome binding site.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Characterization
To characterize this RNA thermometer in vivo, we made an RNA thermometer testing circuit, transformed it into E. coli, and tested the fluorescent output at different temperatures. These graphs show the relative fluorescent intensity of each thermometer between either 25 °C and 30 °C or 25 °C and 37 °C
Figure 1.RNA thermometer testing circuit
Figure 2. Fluorescence intensity at 25 °C, 30 °C, and 37 °C.