Difference between revisions of "Part:BBa K3629009:Design"
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===Design Notes=== | ===Design Notes=== | ||
− | Codon optimized for expression and function in <i>Yarrowia lipolytica.</i> | + | Codon optimized for expression and function in <i>Yarrowia lipolytica.</I> The native signal peptide from <I>T. reesei</i> was also removed so it would not interfere with fused secretion tags native to <i>Y. lipolytica</i> |
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===Source=== | ===Source=== |
Latest revision as of 02:46, 19 October 2020
Trichoderma reesei EGII with FLAG tag
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 283
Illegal NgoMIV site found at 817
Illegal NgoMIV site found at 1135 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
Codon optimized for expression and function in Yarrowia lipolytica. The native signal peptide from T. reesei was also removed so it would not interfere with fused secretion tags native to Y. lipolytica
Source
From genomic sequence of Trichoderma reesei.