Difference between revisions of "Part:BBa K3629002:Design"
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===Design Notes===
 
===Design Notes===
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N/A
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There is a SpeI site within this promoter sequence, making it RFC10 incompatible. However, we added the BioBrick prefix and suffix so that the other enzymes (EcoRI, NotI, XbaI, and PstI) can be used to clone this part into an iGEM plasmid or another plasmid. This part can also be cloned through RFC1000 assembly.
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===Source===
 
===Source===
  
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From genomic sequence of wild-type <i>Y. lipolytica</i> strain CLIB122.
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From genomic sequence of wild-type <i>Y. lipolytica</i> strain CLIB122 CDS: T01033..
  
 
===References===
 
===References===
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1. Blazeck, J., Liu, L., Redden, H., & Alper, H. (2011). Tuning gene expression in Yarrowia lipolytica by a hybrid promoter approach. Applied and environmental microbiology, 77(22), 7905–7914. https://doi.org/10.1128/AEM.05763-11

Latest revision as of 06:27, 26 October 2020


Yarrowia lipolytica EXP Promoter


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 1
    Illegal suffix found in sequence at 1025
    Illegal SpeI site found at 602
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1
    Illegal NheI site found at 663
    Illegal SpeI site found at 602
    Illegal SpeI site found at 1026
    Illegal PstI site found at 1040
    Illegal NotI site found at 7
    Illegal NotI site found at 1033
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 1
    Illegal suffix found in sequence at 1026
    Illegal SpeI site found at 602
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 1
    Illegal XbaI site found at 16
    Illegal SpeI site found at 602
    Illegal SpeI site found at 1026
    Illegal PstI site found at 1040
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

There is a SpeI site within this promoter sequence, making it RFC10 incompatible. However, we added the BioBrick prefix and suffix so that the other enzymes (EcoRI, NotI, XbaI, and PstI) can be used to clone this part into an iGEM plasmid or another plasmid. This part can also be cloned through RFC1000 assembly.

Source

From genomic sequence of wild-type Y. lipolytica strain CLIB122 CDS: T01033..

References

1. Blazeck, J., Liu, L., Redden, H., & Alper, H. (2011). Tuning gene expression in Yarrowia lipolytica by a hybrid promoter approach. Applied and environmental microbiology, 77(22), 7905–7914. https://doi.org/10.1128/AEM.05763-11