Difference between revisions of "Part:BBa K3629007:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | + | Codon optimized for expression and function in <i>Yarrowia lipolytica.</I> The native signal peptide from <I>T. reesei</i> was also removed so it would not interfere with fused secretion tags native to <i>Y. lipolytica</i> | |
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===Source=== | ===Source=== | ||
| − | From genomic sequence of <i> | + | From genomic sequence of <i>Trichoderma reesei</i>. |
===References=== | ===References=== | ||
Latest revision as of 02:47, 19 October 2020
Trichoderma reesei CBHII with FLAG tag
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 341
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 601
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Codon optimized for expression and function in Yarrowia lipolytica. The native signal peptide from T. reesei was also removed so it would not interfere with fused secretion tags native to Y. lipolytica
Source
From genomic sequence of Trichoderma reesei.