Difference between revisions of "Part:BBa K3610044:Design"
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===Design Notes=== | ===Design Notes=== | ||
Codon optimization was done with SnapGene | Codon optimization was done with SnapGene | ||
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===Source=== | ===Source=== | ||
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===References=== | ===References=== | ||
+ | Kunze, Gernot; Zipfel, Cyril; Robatzek, Silke; Niehaus, Karsten; Boller, Thomas; Felix, Georg (2004): The N terminus of bacterial elongation factor Tu elicits innate immunity in Arabidopsis plants. In: Plant Cell 16 (12), S. 3496–3507. DOI: 10.1105/tpc.104.026765. | ||
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+ | England, Christopher G.; Ehlerding, Emily B.; Cai, Weibo (2016): NanoLuc: A Small Luciferase Is Brightening Up the Field of Bioluminescence. In: Bioconjug Chem 27 (5), S. 1175–1187. DOI: 10.1021/acs.bioconjchem.6b00112. | ||
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+ | Barahimipour, Rouhollah; Strenkert, Daniela; Neupert, Juliane; Schroda, Michael; Merchant, Sabeeha S.; Bock, Ralph (2015): Dissecting the contributions of GC content and codon usage to gene expression in the model alga Chlamydomonas reinhardtii. In: Plant J 84 (4), S. 704–717. DOI: 10.1111/tpj.13033. |
Latest revision as of 20:56, 15 October 2020
EFR ectodomain / SmallBit NanoLuc for C. reinhardtii
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 93
Illegal NheI site found at 235
Illegal NheI site found at 1165
Illegal NheI site found at 2083 - 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 148
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1751
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Codon optimization was done with SnapGene
Source
BBa_K2136017, BBa_K3610012, BBa_K3610008, BBa_M10019, BBa_K416001, BBa_K3610017
References
Kunze, Gernot; Zipfel, Cyril; Robatzek, Silke; Niehaus, Karsten; Boller, Thomas; Felix, Georg (2004): The N terminus of bacterial elongation factor Tu elicits innate immunity in Arabidopsis plants. In: Plant Cell 16 (12), S. 3496–3507. DOI: 10.1105/tpc.104.026765.
England, Christopher G.; Ehlerding, Emily B.; Cai, Weibo (2016): NanoLuc: A Small Luciferase Is Brightening Up the Field of Bioluminescence. In: Bioconjug Chem 27 (5), S. 1175–1187. DOI: 10.1021/acs.bioconjchem.6b00112.
Barahimipour, Rouhollah; Strenkert, Daniela; Neupert, Juliane; Schroda, Michael; Merchant, Sabeeha S.; Bock, Ralph (2015): Dissecting the contributions of GC content and codon usage to gene expression in the model alga Chlamydomonas reinhardtii. In: Plant J 84 (4), S. 704–717. DOI: 10.1111/tpj.13033.