Difference between revisions of "Part:BBa K3629001:Design"

 
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===Design Notes===
 
===Design Notes===
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N/A
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The wild-type sequence of this promoter contains BsaI, PstI, and SpeI restriction sites in the promoter section before the intron sequence. Therefore, single-nucleotides changes that match the ones in the functional [https://parts.igem.org/Part:BBa_K2983050 BBa_K2983050] promoter were made to remove these sites.
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===Source===
 
===Source===
  
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From the genomic sequence of Wild-type Yarrowia lipolytica.
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From the genomic sequence of wild-type <i>Y. lipolytica</i> strain CLIB122 CDS: T01033..
  
 
===References===
 
===References===
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1. Blazeck, J., Liu, L., Redden, H., & Alper, H. (2011). Tuning gene expression in Yarrowia lipolytica by a hybrid promoter approach. Applied and environmental microbiology, 77(22), 7905–7914. https://doi.org/10.1128/AEM.05763-11

Latest revision as of 06:14, 26 October 2020


Yarrowia lipolytica TEF1 promoter with an intron


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The wild-type sequence of this promoter contains BsaI, PstI, and SpeI restriction sites in the promoter section before the intron sequence. Therefore, single-nucleotides changes that match the ones in the functional BBa_K2983050 promoter were made to remove these sites.

Source

From the genomic sequence of wild-type Y. lipolytica strain CLIB122 CDS: T01033..

References

1. Blazeck, J., Liu, L., Redden, H., & Alper, H. (2011). Tuning gene expression in Yarrowia lipolytica by a hybrid promoter approach. Applied and environmental microbiology, 77(22), 7905–7914. https://doi.org/10.1128/AEM.05763-11