Difference between revisions of "Part:BBa K3487006"

 
 
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<partinfo>BBa_K3487006 short</partinfo>
 
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===Description===
 
BrnT as a non-specific endonuclease nucleic acid binding mRNA can affect the translation, leading to cell death can not express the normal protein.
 
BrnT as a non-specific endonuclease nucleic acid binding mRNA can affect the translation, leading to cell death can not express the normal protein.
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[[File:T--SZPT-CHINA--BrnT.png|400px|center|pET28a(+), vector positive control ddH2O, negative control RalR, Non-specific endonuclease]]
 
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===Usage and Biology===
 
===Usage and Biology===
  
 
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<span class='h3bb'>Sequence and Features</span>
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===Sequence and Features===
 
<partinfo>BBa_K3487006 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K3487006 SequenceAndFeatures</partinfo>
  
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===References===
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Heaton Brook E, Herrou Julien, Blackwell Anne E, et al. Molecular structure and function of the novel BrnT/BrnA toxin-antitoxin system of Brucella abortus.. 2012, 287(15):12098-110.
 
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===Functional Parameters===
 
===Functional Parameters===
 
<partinfo>BBa_K3487006 parameters</partinfo>
 
<partinfo>BBa_K3487006 parameters</partinfo>
 
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Latest revision as of 12:27, 18 October 2020


Non-specific endonuclease

Description

BrnT as a non-specific endonuclease nucleic acid binding mRNA can affect the translation, leading to cell death can not express the normal protein.

pET28a(+), vector positive control ddH2O, negative control RalR, Non-specific endonuclease

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

References

Heaton Brook E, Herrou Julien, Blackwell Anne E, et al. Molecular structure and function of the novel BrnT/BrnA toxin-antitoxin system of Brucella abortus.. 2012, 287(15):12098-110.