Difference between revisions of "Part:BBa K3408009:Design"

 
 
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===Design Notes===
 
===Design Notes===
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We used this part to verify the feasibility of our IPTG induction system in our engineered <i>Bacillus subtilis</i>.  
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We wanted to make sure that our toehold switch could work normally by checking if there was GFP expressed in the engineered bacteria.
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===Source===
 
===Source===
  
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We designed it by combining some basic parts.
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We designed it with our basic part trigger RNA (BBa_K3408002) and switch RNA (BBa_K3408003).
  
 
===References===
 
===References===

Latest revision as of 02:33, 28 October 2020


PliaG-trigger RNA-B0015-PCⅠ-switch RNA-GFP-B0015


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 239
    Illegal BglII site found at 461
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1190


Design Notes

We wanted to make sure that our toehold switch could work normally by checking if there was GFP expressed in the engineered bacteria.

Source

We designed it with our basic part trigger RNA (BBa_K3408002) and switch RNA (BBa_K3408003).

References