Difference between revisions of "Part:BBa K3519003"

 
 
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<partinfo>BBa_K3519003 short</partinfo>
 
<partinfo>BBa_K3519003 short</partinfo>
  
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This part codes for the gene traT. The genes traS and traT help in surface exclusion and are carried naturally by the E. coli F factor.  
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This part codes for traT. The genes traS and traT help in surface exclusion and are carried naturally by the E. coli Fertiity factor (F factor). TraT is an outer membrane protein that prevents reception of conjugal tubes. The simultaneous overexpression of both these genes in E. coli has been shown to reduce DNA transfer by 33,000 times. Thus, they help reduce conjugation significantly.
  
 
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===Usage and Biology===
 
===Usage and Biology===
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Latest revision as of 13:16, 24 October 2020


traT

This part codes for traT. The genes traS and traT help in surface exclusion and are carried naturally by the E. coli Fertiity factor (F factor). TraT is an outer membrane protein that prevents reception of conjugal tubes. The simultaneous overexpression of both these genes in E. coli has been shown to reduce DNA transfer by 33,000 times. Thus, they help reduce conjugation significantly.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 642
    Illegal BsaI.rc site found at 124