Difference between revisions of "Part:BBa K3408004"
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<partinfo>BBa_K3408004 short</partinfo> | <partinfo>BBa_K3408004 short</partinfo> | ||
− | It is a protein which could suppress | + | It is a protein which could suppress P<sub>CⅠ</sub> so that our switch RNA couldn't be transcribed without oxygen. |
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+ | We designed it under the control of P<sub>nar</sub>. | ||
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+ | We have optimized the CⅠ protein on ThermoFisher SCIENTIFIC (https://www.thermofisher.com/cn/zh/home/life-science/cloning/gene-synthesis/geneart-gene-synthesis/geneoptimizer.html) to use it better in our engineered bacteria. | ||
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+ | https://2020.igem.org/wiki/images/4/43/T--NAU-CHINA--basic-parts3.png | ||
+ | https://2020.igem.org/wiki/images/5/5e/T--NAU-CHINA--basic-parts4.png | ||
+ | |||
+ | Fig.1. Codon quality distribution Fig.2. Optimized codon quality distribution | ||
+ | |||
+ | |||
+ | The histograms show the percentage of sequence codons which fall into a certain quality class. The quality value of the most frequently used codon for a given amino acid in the desired expression system is set to 100, the remaining codons are scaled accordingly (Sharp, P.M., Li, W.H., <i>Nucleic Acids Res</i>. 15 (3),1987). | ||
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+ | https://2020.igem.org/wiki/images/b/bf/T--NAU-CHINA--basic-parts5.png | ||
+ | https://2020.igem.org/wiki/images/7/77/T--NAU-CHINA--basic-parts6.png | ||
+ | |||
+ | Fig.3. Codon quality plot Fig.4. Optimized codon quality plot | ||
+ | |||
+ | The plots show the quality of the used codon at the indicated codon position. | ||
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<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
===Usage and Biology=== | ===Usage and Biology=== |
Latest revision as of 01:35, 28 October 2020
Optimized CⅠ
It is a protein which could suppress PCⅠ so that our switch RNA couldn't be transcribed without oxygen.
We designed it under the control of Pnar.
We have optimized the CⅠ protein on ThermoFisher SCIENTIFIC (https://www.thermofisher.com/cn/zh/home/life-science/cloning/gene-synthesis/geneart-gene-synthesis/geneoptimizer.html) to use it better in our engineered bacteria.
Fig.1. Codon quality distribution Fig.2. Optimized codon quality distribution
The histograms show the percentage of sequence codons which fall into a certain quality class. The quality value of the most frequently used codon for a given amino acid in the desired expression system is set to 100, the remaining codons are scaled accordingly (Sharp, P.M., Li, W.H., Nucleic Acids Res. 15 (3),1987).
Fig.3. Codon quality plot Fig.4. Optimized codon quality plot
The plots show the quality of the used codon at the indicated codon position.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]