Difference between revisions of "Part:BBa K3376001"
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<partinfo>BBa_K3376001 short</partinfo> | <partinfo>BBa_K3376001 short</partinfo> | ||
− | The basic part of | + | The basic part of GFP was modified from GFPmut1 ([https://parts.igem.org/Part:BBa_K1159311 Part:BBa_K1159311]) designed by TU-Munich in iGEM 2013 by adding ATG and a stop codon for protein expression. The GFPmut1 was assembled with a double terminator. |
=== Expression === | === Expression === | ||
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− | <span class='h3bb'>Sequence and Features</span> | + | ===<span class='h3bb'>Sequence and Features</span>=== |
<partinfo>BBa_K3376001 SequenceAndFeatures</partinfo> | <partinfo>BBa_K3376001 SequenceAndFeatures</partinfo> | ||
Latest revision as of 07:11, 6 October 2020
GFP-Tr/pSB1C3
The basic part of GFP was modified from GFPmut1 (Part:BBa_K1159311) designed by TU-Munich in iGEM 2013 by adding ATG and a stop codon for protein expression. The GFPmut1 was assembled with a double terminator.
Expression
GFP was detected at Ex/Em = 483/513. The fluorescence expression levels were measured for lactate dehydrogenase promoter (ldhp) [ldhp-GFP-Tr/pSB1C3 (BBa_K3376002)] and thiol peroxidase promoter (tpxp) [tpxp-GFP-Tr/pSB1C3 (BBa_K3376004)] activity of S. mutans.
Transformation
The ldhp-GFP-Tr/pSB1C3 [BBa_K3376002] was transformed into E. coli Nissle strain. The high expression of green fluorescent protein was observed under a blue led light.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 644