Difference between revisions of "Part:BBa K3376014"
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=== Expression === | === Expression === | ||
| − | GFP was detected at Ex/Em = 483/513. The fluorescence expression levels were measured for lactate dehydrogenase promoter (ldhp) [ldhp-GFP-Tr/pSB1C3 (BBa_K3376002)] and thiol peroxidase promoter (tpxp)[tpxp-GFP-Tr/pSB1C3 (BBa_K3376004)] activity of S. mutans. | + | GFP was detected at Ex/Em = 483/513. The fluorescence expression levels were measured for lactate dehydrogenase promoter (ldhp) [ldhp-GFP-Tr/pSB1C3 (BBa_K3376002)] and thiol peroxidase promoter (tpxp) [tpxp-GFP-Tr/pSB1C3 (BBa_K3376004)] activity of S. mutans. |
| − | [[File:T--Mingdao--ww1.png| | + | [[File:T--Mingdao--ww1.png|450px|center]] |
=== Transformation === | === Transformation === | ||
The ldhp-GFP-Tr/pSB1C3 [BBa_K3376002] was transformed into E. coli Nissle strain. The high expression of green fluorescent protein was observed under a blue led light. | The ldhp-GFP-Tr/pSB1C3 [BBa_K3376002] was transformed into E. coli Nissle strain. The high expression of green fluorescent protein was observed under a blue led light. | ||
| − | [[File:T--Mingdao--ww2.png| | + | [[File:T--Mingdao--ww2.png|600px|center]] |
Latest revision as of 04:57, 6 October 2020
Aequorea victoria GFPmut1
This part was modified from GFPmut1 (Part:BBa_K1159311) designed by TU-Munich in iGEM 2013 by adding ATG and a stop codon for protein expression.
Expression
GFP was detected at Ex/Em = 483/513. The fluorescence expression levels were measured for lactate dehydrogenase promoter (ldhp) [ldhp-GFP-Tr/pSB1C3 (BBa_K3376002)] and thiol peroxidase promoter (tpxp) [tpxp-GFP-Tr/pSB1C3 (BBa_K3376004)] activity of S. mutans.
Transformation
The ldhp-GFP-Tr/pSB1C3 [BBa_K3376002] was transformed into E. coli Nissle strain. The high expression of green fluorescent protein was observed under a blue led light.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 644