Difference between revisions of "Part:BBa K3329000"
 
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<partinfo>BBa_K3329000 short</partinfo>
 
<partinfo>BBa_K3329000 short</partinfo>
  
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This is a sub part in the Spytag-catcher003 system. It should be used with Spycatcher003.
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This is a sub part in the SpyTag003-SpyCatcher003 system. It should be used with SpyCatcher003. It's noteworthy that, in our Protein Crosslinking design, we intended to link a triplication of SpyTag003 through elastin-like polypeptide. Moreover, SpyTag003 was expressed in both P. Pastoris and E. coli, respectively. Therefore, codon optimization must be applied in order to avoid homologous recombination and fit the chassis organism. As a result, we synthesized 9 versions of genes coding the same SpyTag003 sequence based on the codon optimization of P. Pastoris and 3 versions for the codon optimization of E. coli. Names for those 12 versions of CDS is documented from SpyTag003 optimized V1 to SpyTag003 optimized V12, in which the SpyTag003 optimized V1-V6 are for P. Pastoris, and SpyTag003 optimized V7-V12 are for E. coli.
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We also built a model calculating the optimized ratio of SpyCatcher003 to SpyTag003 for gel formation. See more details in our Engineering Success webpage: https://2020.igem.org/Team:BNDS_China/Engineering
  
 
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===References===
 
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Functional Parameters
 
 
<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K3329000 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K3329000 SequenceAndFeatures</partinfo>
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===References===
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Functional Parameters
 
<partinfo>BBa_K3329000 parameters</partinfo>
 
<partinfo>BBa_K3329000 parameters</partinfo>
 
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Latest revision as of 09:26, 27 October 2020


SpyTag003 optimized V1

This is a sub part in the SpyTag003-SpyCatcher003 system. It should be used with SpyCatcher003. It's noteworthy that, in our Protein Crosslinking design, we intended to link a triplication of SpyTag003 through elastin-like polypeptide. Moreover, SpyTag003 was expressed in both P. Pastoris and E. coli, respectively. Therefore, codon optimization must be applied in order to avoid homologous recombination and fit the chassis organism. As a result, we synthesized 9 versions of genes coding the same SpyTag003 sequence based on the codon optimization of P. Pastoris and 3 versions for the codon optimization of E. coli. Names for those 12 versions of CDS is documented from SpyTag003 optimized V1 to SpyTag003 optimized V12, in which the SpyTag003 optimized V1-V6 are for P. Pastoris, and SpyTag003 optimized V7-V12 are for E. coli.

We also built a model calculating the optimized ratio of SpyCatcher003 to SpyTag003 for gel formation. See more details in our Engineering Success webpage: https://2020.igem.org/Team:BNDS_China/Engineering

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


References

Functional Parameters