Difference between revisions of "Part:BBa K123003"

 
 
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<partinfo>BBa_K123003 short</partinfo>
 
<partinfo>BBa_K123003 short</partinfo>
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[[image:ER.jpg]]<br>
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This basic part is derived from the Human Estrogen Receptor Alpha and optimized for activity in E.Coli. When estrogen or an estrogen like compound is present the receptor binds to it and forms a homodimer. This homodimer is then able to bind to an Estrogen Responsive Element (ERE BBA_K123002). In Humans this estrogen receptor (ER) is a transcription factor that inciates transcription. However because we are interested in using this protien in bacteria we could not assume the same enhancer like effects. We have designed this part to act as a repressor when bound to the ERE by physically blocking the Polymerase
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This basic part is derived from the Human Estrogen Receptor Alpha and optimized for activity in E. coli. When estrogen or an estrogen like compound is present the receptor binds to it and forms a homodimer. This homodimer is then able to bind to an Estrogen Responsive Element (ERE BBA_K123002). In humans this estrogen receptor (ER) is a transcription factor that initiates transcription. However because we are interested in using this protein in bacteria we could not assume the same enhancer like effects. We have therefore designed this part to act as a repressor when bound to the ERE by physically blocking the polymerase.
  
 
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===Usage and Biology===
 
  
 
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<span class='h3bb'>Sequence and Features</span>
 
 
<partinfo>BBa_K123003 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K123003 SequenceAndFeatures</partinfo>
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===Usage and Biology===
  
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[[image:toxicity.jpg]]<br>
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'''Figure 1:'''<br>
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This figure depicts the effect that ER has on the growth rate of a cell quantified by optical density of the culture at 600nm. Our control was a strain containing an empty I0500 plasmid. ER1 is the DNA binding domain while ER2 is the ligand binding domain. We separated these to determine the toxicity of each domain.
  
 
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Latest revision as of 20:58, 29 October 2008

ER ER.jpg
This basic part is derived from the Human Estrogen Receptor Alpha and optimized for activity in E. coli. When estrogen or an estrogen like compound is present the receptor binds to it and forms a homodimer. This homodimer is then able to bind to an Estrogen Responsive Element (ERE BBA_K123002). In humans this estrogen receptor (ER) is a transcription factor that initiates transcription. However because we are interested in using this protein in bacteria we could not assume the same enhancer like effects. We have therefore designed this part to act as a repressor when bound to the ERE by physically blocking the polymerase.


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 24
    Illegal NgoMIV site found at 902
    Illegal AgeI site found at 870
    Illegal AgeI site found at 1841
  • 1000
    COMPATIBLE WITH RFC[1000]

Usage and Biology

Toxicity.jpg
Figure 1:
This figure depicts the effect that ER has on the growth rate of a cell quantified by optical density of the culture at 600nm. Our control was a strain containing an empty I0500 plasmid. ER1 is the DNA binding domain while ER2 is the ligand binding domain. We separated these to determine the toxicity of each domain.