Difference between revisions of "Part:BBa K123001"

 
 
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<partinfo>BBa_K123001 short</partinfo>
 
<partinfo>BBa_K123001 short</partinfo>
  
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When used in conjunction with BisdA this enzyme can be used to degrade Bisphenol A. Activity has previously been recorded in E.coli by Miho Sasaki1 et al. 2005.  
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When used in conjunction with BisdA this enzyme can be used to degrade Bisphenol A. Activity has previously been recorded in E.coli.
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<[[image:bpa.jpeg]]>
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'''Figure 3''' BPA degradation by bisdB- and bisdAB-recombinant cells in L-BPA medium. The symbols represent the BPA content remaining in L-BPA medium in which E. coli BL21 (DE3) cells bearing pET17b (circles), pET17bisdB (triangles), and pET17bisdAB (squares) were grown. Error bars indicate standard deviations from three independent experiments.
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Figure Taken From:
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M. Sasaki, T. Tsuchido, Y. Matsumura. 2008 Molecular cloning and characterization of cytochrome P450 and ferredoxin <br>genes involved in bisphenol A degradation in Sphingomonas bisphenolicum strain AO1. Journal of Applied Microbiology <br> 105:4:1158-1169
  
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Miho Sasaki1, Jun-ichi Maki, Ko-ichi Oshiman, Yoshinobu Matsumura1, and Tetsuaki Tsuchido.2005, Biodegradation of bisphenol A by cells and cell lysate from Sphingomonas sp. strain AO1. Biodegredation 16: 449-459
 
  
  

Latest revision as of 03:09, 29 October 2008

BisdB

When used in conjunction with BisdA this enzyme can be used to degrade Bisphenol A. Activity has previously been recorded in E.coli.


<Bpa.jpeg>


Figure 3 BPA degradation by bisdB- and bisdAB-recombinant cells in L-BPA medium. The symbols represent the BPA content remaining in L-BPA medium in which E. coli BL21 (DE3) cells bearing pET17b (circles), pET17bisdB (triangles), and pET17bisdAB (squares) were grown. Error bars indicate standard deviations from three independent experiments. Figure Taken From: 

M. Sasaki, T. Tsuchido, Y. Matsumura. 2008 Molecular cloning and characterization of cytochrome P450 and ferredoxin 
genes involved in bisphenol A degradation in Sphingomonas bisphenolicum strain AO1. Journal of Applied Microbiology 
 105:4:1158-1169


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 479
    Illegal BamHI site found at 867
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 4
    Illegal AgeI site found at 1276
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1086