Difference between revisions of "Part:BBa K3408000:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | The spacer sequence of 15 bp between the - 35 and - 10 elements of the upstream region of the wild-type nar promoter was randomized. | + | The spacer sequence of 15 bp between the -35 and -10 elements of the upstream region of the wild-type nar promoter was randomized. |
| − | + | ||
| − | + | ||
===Source=== | ===Source=== | ||
| − | The sequence is found through the paper | + | The sequence is found through the paper 'Engineering and application of synthetic nar promoter for fne-tuning the expression of metabolic pathway genes in Escherichia coli'. |
===References=== | ===References=== | ||
| − | + | [1] Hwang Hee Jin,Lee Sang Yup,Lee Pyung Cheon. Engineering and application of synthetic nar promoter for fine-tuning the expression of metabolic pathway genes in Escherichia coli [J]. Biotechnology for biofuels,2018,11. | |
Latest revision as of 01:15, 28 October 2020
promoter nar(fnr regulated)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The spacer sequence of 15 bp between the -35 and -10 elements of the upstream region of the wild-type nar promoter was randomized.
Source
The sequence is found through the paper 'Engineering and application of synthetic nar promoter for fne-tuning the expression of metabolic pathway genes in Escherichia coli'.
References
[1] Hwang Hee Jin,Lee Sang Yup,Lee Pyung Cheon. Engineering and application of synthetic nar promoter for fine-tuning the expression of metabolic pathway genes in Escherichia coli [J]. Biotechnology for biofuels,2018,11.