Difference between revisions of "Part:BBa K3349002:Design"

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===Design Notes===
 
===Design Notes===
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This part was codon optimized for use in <i> E. coli </i>. A C-terminal His tag was added based on previous pectin lyase research [2].
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This part was codon optimized for use in <i> E. coli</i>. A C-terminal His tag was added based on previous pectin lyase research [2].
  
 
===Source===
 
===Source===
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===References===
 
===References===
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1. Mie, Y., Chen, Y., Zhai, R., and Liu, Y. (2013) Cloning, purification, and biochemical properties of a thermostable pectinase <i> Bacillus halodurans </i> M29. Journal of Molecular catalysis B: Enzymatic. 94, 77-81
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1.  Keggi, C and Doran-Peterson, J. (2020) The homogalacturonan deconstruction system of <i>Paenibacillus amylolyticus</i> 27C64 requires no extracellular Pectin Methylesterase and has significant industrial potential. Applied and environmental microbiology. 86, e02275-19.
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2. Mie, Y., Chen, Y., Zhai, R., and Liu, Y. (2013) Cloning, purification, and biochemical properties of a thermostable pectinase <i> Bacillus halodurans </i> M29. Journal of Molecular catalysis B: Enzymatic. 94, 77-81

Latest revision as of 15:07, 7 October 2020


Pectin Lyase PelC


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 829
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 215


Design Notes

This part was codon optimized for use in E. coli. A C-terminal His tag was added based on previous pectin lyase research [2].

Source

This part was originally taken from the uniprot database. The NCBI sequence ID is WP_123062310.1.

References

1. Keggi, C and Doran-Peterson, J. (2020) The homogalacturonan deconstruction system of Paenibacillus amylolyticus 27C64 requires no extracellular Pectin Methylesterase and has significant industrial potential. Applied and environmental microbiology. 86, e02275-19.

2. Mie, Y., Chen, Y., Zhai, R., and Liu, Y. (2013) Cloning, purification, and biochemical properties of a thermostable pectinase Bacillus halodurans M29. Journal of Molecular catalysis B: Enzymatic. 94, 77-81