Difference between revisions of "Part:BBa K103014:Design"

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===Design Notes===
 
===Design Notes===
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[http://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=3&arg0=20_October_2008&arg1=21_October_2008&arg2=22_October_2008&name=Preparation%20of%20OmpA_linker_alpha_linker%20(BBa_K103014) Preparation of OmpA-linker-alpha-linker]
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Preparation of BBa_K103014 is in details described [http://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=3&arg0=20_October_2008&arg1=21_October_2008&arg2=22_October_2008&name=Preparation%20of%20OmpA_linker_alpha_linker%20(BBa_K103014) here] (entries from Univeristy of Warsaw 2008 iGEM team notebook).
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[https://parts.igem.org/Part:BBa_K103006 BBa_K103006] was digested with EcoRI and SacI and ligated into [https://parts.igem.org/Part:pSB2K3 pSB2K3] standard BioBrick vector ([https://parts.igem.org/Part:pSB2K3 pSB2K3] with SacI site obtained by EcoRI/SacI digest of standard BioBrick vector [https://parts.igem.org/Part:pSB2K3 pSB2K3] carrying [https://parts.igem.org/Part:BBa_K103017 BBa_K103017] part)
  
 
===Source===
 
===Source===

Latest revision as of 14:26, 28 October 2008

OmpA_linker_alpha_linker


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1042
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Preparation of BBa_K103014 is in details described [http://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=3&arg0=20_October_2008&arg1=21_October_2008&arg2=22_October_2008&name=Preparation%20of%20OmpA_linker_alpha_linker%20(BBa_K103014) here] (entries from Univeristy of Warsaw 2008 iGEM team notebook).

BBa_K103006 was digested with EcoRI and SacI and ligated into pSB2K3 standard BioBrick vector (pSB2K3 with SacI site obtained by EcoRI/SacI digest of standard BioBrick vector pSB2K3 carrying BBa_K103017 part)

Source

References