Difference between revisions of "Part:BBa K3447002"
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− | + | Axh43 gene, also called xynD, is derived from <i>Bacillus subtilis</i> 168 and belongs to the GH43 hydrolase family. It can specifically hydrolyze the Arabinose residues on the O-2 xylan or o-3 xylan, and assist the hydrolysis of the xylan. | |
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===Usage and Biology=== | ===Usage and Biology=== | ||
− | + | In our project, we use this part together with xynA gene <partinfo>BBa_K1175005</Partinfo> to hydrolyze xylan and produce arabinose. For more information, please check out our part <partinfo>BBa_K3447102</Partinfo>. | |
− | We | + | |
+ | ==<b>Design</b>== | ||
+ | ===Design Notes=== | ||
+ | There is no need to pay attention.<br> | ||
+ | |||
+ | ===Source=== | ||
+ | |||
+ | This gene is derived from <i>Bacillus subtilis</i> 168, we get the genomic sequence by NCBI.<br> | ||
+ | We ordered the part DNA from a synthesis company.<br> | ||
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− | <span class='h3bb' | + | <span class='h3bb'>Sequence and Features</span> |
<partinfo>BBa_K3447002 SequenceAndFeatures</partinfo> | <partinfo>BBa_K3447002 SequenceAndFeatures</partinfo> | ||
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<partinfo>BBa_K3447002 parameters</partinfo> | <partinfo>BBa_K3447002 parameters</partinfo> | ||
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+ | |||
+ | |||
+ | |||
+ | ===References=== |
Latest revision as of 10:20, 27 October 2020
xynD, which produces xylanase
Axh43 gene, also called xynD, is derived from Bacillus subtilis 168 and belongs to the GH43 hydrolase family. It can specifically hydrolyze the Arabinose residues on the O-2 xylan or o-3 xylan, and assist the hydrolysis of the xylan.
Usage and Biology
In our project, we use this part together with xynA gene BBa_K1175005 to hydrolyze xylan and produce arabinose. For more information, please check out our part BBa_K3447102.
Design
Design Notes
There is no need to pay attention.
Source
This gene is derived from Bacillus subtilis 168, we get the genomic sequence by NCBI.
We ordered the part DNA from a synthesis company.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 712