Difference between revisions of "Part:BBa K3406003"
 
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<partinfo>BBa_K3406003 short</partinfo>
 
<partinfo>BBa_K3406003 short</partinfo>
  
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The coding sequence of LbaCas13a protein.It contains rare codons, so it needs to be expressed in <i>E. coli</i> strains which is rich in corresponding tRNAs like <i>E. coli</i> Rosetta2(DE3)pLysS.
 
<partinfo>BBa_K3406003 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K3406003 SequenceAndFeatures</partinfo>

Latest revision as of 06:23, 27 October 2020

LbaCas13a coding sequence

The coding sequence of LbaCas13a protein.It contains rare codons, so it needs to be expressed in E. coli strains which is rich in corresponding tRNAs like E. coli Rosetta2(DE3)pLysS.


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2168
    Illegal BglII site found at 3683
    Illegal BamHI site found at 95
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1738
    Illegal AgeI site found at 2815
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 409
    Illegal BsaI.rc site found at 1492