Difference between revisions of "Part:BBa K3417000"
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This part is the coding sequence of the protein Phaselotoxin Resistant Ornithine Carbamoyltransferase (ROTCase), which substitutes the metabolic role of <i>E. coli</i> ornithine carbamoyltransferase in the bacterial urea cycle.
 
This part is the coding sequence of the protein Phaselotoxin Resistant Ornithine Carbamoyltransferase (ROTCase), which substitutes the metabolic role of <i>E. coli</i> ornithine carbamoyltransferase in the bacterial urea cycle.
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https://static.igem.org/mediawiki/parts/5/58/T--Gaston_Day_School--sequential.png
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This figure shows the Sequential Bi-Bi Mechanism of ROTCase, in which carbomylphosphate is the first substrate to bind, allowing ornithine to then bind to the complex.
 
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===Usage and Biology===
 
===Usage and Biology===
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===Functional Parameters===
 
===Functional Parameters===
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<partinfo>BBa_K3417000 parameters</partinfo>
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kcat = 2940 min^(-1)
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===References===
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Kelly, J., Rubin, A., Davis, J., Ajo-franklin, C., Cumbers, J., Czar, M., De Mora, K., Glieberman, A., Monie, D., &Endy, D. (2009). Measuring the activity of biobrick promoters using an in vivo reference standard.Journalof Biological Engineering,3(1), 4. https://doi.org/10.1186/1754-1611-3-4
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Templeton, M. D., Reinhardt, L. A., Collyer, C. A., Mitchell, R. E., & Cleland, W. W. (2005). Kinetic analysisof the l-ornithine transcarbamoylase from pseudomonas savastanoi pv. phaseolicola that is resistant to thetransition  state  analogue  (r)-n-(n‘-sulfodiaminophosphinyl)-l-ornithine  [PMID:  15766270].Biochemistry,44(11), 4408–4415. https://doi.org/10.1021/bi047432x

Latest revision as of 00:49, 28 October 2020


ArgK Coding Sequence

This part is the coding sequence of the protein Phaselotoxin Resistant Ornithine Carbamoyltransferase (ROTCase), which substitutes the metabolic role of E. coli ornithine carbamoyltransferase in the bacterial urea cycle. T--Gaston_Day_School--sequential.png This figure shows the Sequential Bi-Bi Mechanism of ROTCase, in which carbomylphosphate is the first substrate to bind, allowing ornithine to then bind to the complex. Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 859


Functional Parameters

kcat = 2940 min^(-1)

References

Kelly, J., Rubin, A., Davis, J., Ajo-franklin, C., Cumbers, J., Czar, M., De Mora, K., Glieberman, A., Monie, D., &Endy, D. (2009). Measuring the activity of biobrick promoters using an in vivo reference standard.Journalof Biological Engineering,3(1), 4. https://doi.org/10.1186/1754-1611-3-4

Templeton, M. D., Reinhardt, L. A., Collyer, C. A., Mitchell, R. E., & Cleland, W. W. (2005). Kinetic analysisof the l-ornithine transcarbamoylase from pseudomonas savastanoi pv. phaseolicola that is resistant to thetransition state analogue (r)-n-(n‘-sulfodiaminophosphinyl)-l-ornithine [PMID: 15766270].Biochemistry,44(11), 4408–4415. https://doi.org/10.1021/bi047432x