Difference between revisions of "Part:BBa K3406001"

 
 
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__NOTOC__
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<partinfo>BBa_K3406001 short</partinfo>
  
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The original CcaCas13b protein gene contains rare codons. We optimized the codon to make it could be expressed in common <i>E. coli</i> protein expression strains, such as <i>E. coli</i> BL21(DE3).
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<partinfo>BBa_K3406001 SequenceAndFeatures</partinfo>

Latest revision as of 06:26, 27 October 2020

CcaCas13b coding sequence

The original CcaCas13b protein gene contains rare codons. We optimized the codon to make it could be expressed in common E. coli protein expression strains, such as E. coli BL21(DE3).


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 954
    Illegal AgeI site found at 1806
  • 1000
    COMPATIBLE WITH RFC[1000]