Difference between revisions of "Part:BBa K3458001:Design"

 
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===Design Notes===
 
===Design Notes===
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* We synthesized this DNA sample through TIANYI HUIYUAN Company.
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In order to have a better expression in ''Oryza sativa L.'', we have carried out codon optimization according to the codon preference of ''Oryza sativa L.''.
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* In order to meet the assembly requirements, we removed the illegal restriction site.
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* In order to express HQT in Oryza sativa L. and conduct further tests, we also designed two composite originals, GluD-1::HQT ([https://parts.igem.org/Part:BBa_K3458004 BBa_K3458004]) and 35S::HQT ([https://parts.igem.org/Part:BBa_K3458003 BBa_K3458003]).
  
 
===Source===
 
===Source===
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The HQT gene we used comes from the genome of ''Lonicera japonica'' (GeneBank:[https://www.ncbi.nlm.nih.gov/nuccore/JF261014.1 JF261014.1]). We removed the restriction site incompatible with the biobrick assembly standard  and optimized the codon according to the codon preference of ''Oryza sativa L.''.The HQT was synthesized in TIANYI HUIYUAN  Company.
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===References===
 
===References===
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Peng X, Li W, Wang W, et al. Cloning and characterization of a cDNA coding a hydroxycinnamoyl-CoA quinate hydroxycinnamoyl transferase involved in chlorogenic acid biosynthesis in Lonicera japonica[J]. Planta Med., 2010,76(16):1921-1926.

Latest revision as of 02:34, 17 August 2020


HQT(Hydroxycinnamoyl-CoA Quinate Hydroxycinnamoyl Transferase)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

  • We synthesized this DNA sample through TIANYI HUIYUAN Company.

In order to have a better expression in Oryza sativa L., we have carried out codon optimization according to the codon preference of Oryza sativa L..

  • In order to meet the assembly requirements, we removed the illegal restriction site.
  • In order to express HQT in Oryza sativa L. and conduct further tests, we also designed two composite originals, GluD-1::HQT (BBa_K3458004) and 35S::HQT (BBa_K3458003).

Source

The HQT gene we used comes from the genome of Lonicera japonica (GeneBank:JF261014.1). We removed the restriction site incompatible with the biobrick assembly standard and optimized the codon according to the codon preference of Oryza sativa L..The HQT was synthesized in TIANYI HUIYUAN Company.

References

Peng X, Li W, Wang W, et al. Cloning and characterization of a cDNA coding a hydroxycinnamoyl-CoA quinate hydroxycinnamoyl transferase involved in chlorogenic acid biosynthesis in Lonicera japonica[J]. Planta Med., 2010,76(16):1921-1926.