Difference between revisions of "Part:BBa J119447"
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| − | The Schistosome Ribozyme is a riboswitch that cleaves itself in the presence of theophylline. It is cloned into the pSB1A2-BR vector, allowing for gene expression using a T7 promoter. | + | The Schistosome Ribozyme is a riboswitch that cleaves itself in the presence of theophylline. It is cloned into the pSB1A2-BR vector, allowing for gene expression using a T7 promoter. The sequence for this ribozyme is found in Computational Design and Biosensor Application of Small Molecule-Sensing Allosteric Ribozymes (https://pubs.acs.org/doi/abs/10.1021/bm400299a). |
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Latest revision as of 18:45, 18 June 2020
Schistosome Ribozyme
The Schistosome Ribozyme is a riboswitch that cleaves itself in the presence of theophylline. It is cloned into the pSB1A2-BR vector, allowing for gene expression using a T7 promoter. The sequence for this ribozyme is found in Computational Design and Biosensor Application of Small Molecule-Sensing Allosteric Ribozymes (https://pubs.acs.org/doi/abs/10.1021/bm400299a).
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]