Difference between revisions of "Template:TypeIIS/Plasmids/Level2"
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</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td></html><partinfo>pSB3C01</partinfo><html></td> | + | <td></html>[[Part:pSB3C01|<partinfo>pSB3C01</partinfo>]]<html></td> |
<td>pEven1</td> | <td>pEven1</td> | ||
<td class="dnaSeq rightAlign">GGAG</td> | <td class="dnaSeq rightAlign">GGAG</td> | ||
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</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td></html><partinfo>pSB3C02</partinfo><html></td> | + | <td></html>[[Part:pSB3C02|<partinfo>pSB3C02</partinfo>]]<html></td> |
<td>pEven2</td> | <td>pEven2</td> | ||
<td class="dnaSeq rightAlign">TACT</td> | <td class="dnaSeq rightAlign">TACT</td> | ||
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</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td></html><partinfo>pSB3C03</partinfo><html></td> | + | <td></html>[[Part:pSB3C03|<partinfo>pSB3C03</partinfo>]]<html></td> |
<td>pEven3</td> | <td>pEven3</td> | ||
<td class="dnaSeq rightAlign">AATG</td> | <td class="dnaSeq rightAlign">AATG</td> | ||
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</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td></html><partinfo>pSB3C04</partinfo><html></td> | + | <td></html>[[Part:pSB3C04|<partinfo>pSB3C04</partinfo>]]<html></td> |
<td>pEven4</td> | <td>pEven4</td> | ||
<td class="dnaSeq rightAlign"> GCTT </td> | <td class="dnaSeq rightAlign"> GCTT </td> | ||
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</html> | </html> | ||
| − | '''Note:''' Currently, Registry samples of these plasmid backbones have not been fully sequenced. Only their prefix and suffix along with their insert <partinfo>BBa_J04455</partinfo> have been verified. | + | '''Note:''' |
| + | *The documented sequence shows an additional BsaI site. This would impact assembly out of (but not into) this set of vectors. A new set of vectors should be created or used in the long term. | ||
| + | *Currently, Registry samples of these plasmid backbones have not been fully sequenced. Only their prefix and suffix along with their insert <partinfo>BBa_J04455</partinfo> have been verified. | ||
Latest revision as of 15:31, 16 June 2020
When four Level 1 parts (TUs) are assembled into a Multi-Transcriptional Unit (MTU) into the following plasmid backbones (with BBa_J04455), the MTU will be flanked by BsaI restriction sites and these 4 bp Fusion Sites.
| Registry Name | Loop Name | Fusion Site 5' | MTU | Fusion Site 3' |
|---|---|---|---|---|
| pSB3C01 | pEven1 | GGAG | MTU 1 | TACT |
| pSB3C02 | pEven2 | TACT | MTU 2 | AATG |
| pSB3C03 | pEven3 | AATG | MTU 3 | GCTT |
| pSB3C04 | pEven4 | GCTT | MTU 4 | CGCT |
Note:
- The documented sequence shows an additional BsaI site. This would impact assembly out of (but not into) this set of vectors. A new set of vectors should be created or used in the long term.
- Currently, Registry samples of these plasmid backbones have not been fully sequenced. Only their prefix and suffix along with their insert BBa_J04455 have been verified.