Difference between revisions of "Part:BBa K3600003:Design"

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===Design Notes===
 
===Design Notes===
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The sequence of 500bp preceding the ORF of GSH1 was retrieved from the yeast genome database. No modifications were applied. The presence of the GSHI yAP-1 response element (YRE) was confirmed by sequence analysis.
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The sequence of 500bp preceding the ORF of GSH1 was retrieved from the yeast genome database. No modifications were applied. The presence of the GSHI yAP-1 response element (YRE) was confirmed by sequence analysis. YRE has been shown to be essential for the transcriptional control of GSH1 by yAP-1.<html><a href="#Wu1994"><sup>1</sup></a></html>
  
 
===Source===
 
===Source===
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===References===
 
===References===
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<HTML><a name=Wu1994 style="color:black">
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[1] Wu, A. L. & Moye-Rowley, W. S. GSH1, which encodes gamma-glutamylcysteine synthetase, is a target gene for yAP-1 transcriptional regulation. Molecular and Cellular Biology 14, 5832–5839 (1994).
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Latest revision as of 19:29, 14 June 2020


GSH1 promoter part plasmid


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 975
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 563
    Illegal BsaI site found at 972
    Illegal BsaI.rc site found at 1084


Design Notes

The sequence of 500bp preceding the ORF of GSH1 was retrieved from the yeast genome database. No modifications were applied. The presence of the GSHI yAP-1 response element (YRE) was confirmed by sequence analysis. YRE has been shown to be essential for the transcriptional control of GSH1 by yAP-1.1

Source

Promoter of the gamma-glutamylcysteine synthetase (YJL101C) from the genome of S. Cerevisiae.

References

[1] Wu, A. L. & Moye-Rowley, W. S. GSH1, which encodes gamma-glutamylcysteine synthetase, is a target gene for yAP-1 transcriptional regulation. Molecular and Cellular Biology 14, 5832–5839 (1994).