Difference between revisions of "Part:BBa K3600000:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | . | + | The sequence of 500bp preceding the ORF of HSP12 was retrieved from the yeast genome database. No modifications were applied. The presence of 5 stress response elements (STRE) was confirmed by sequence analysis. |
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===Source=== | ===Source=== | ||
| − | . | + | Promoter of the small heat-shock protein HSP12 (YFL014W) from the genome of S. Cerevisiae. |
===References=== | ===References=== | ||
Latest revision as of 15:19, 11 June 2020
HSP12 promoter part plasmid
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal XbaI site found at 308
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 326
- 23INCOMPATIBLE WITH RFC[23]Illegal XbaI site found at 308
- 25INCOMPATIBLE WITH RFC[25]Illegal XbaI site found at 308
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 57
Illegal BsaI.rc site found at 578
Design Notes
The sequence of 500bp preceding the ORF of HSP12 was retrieved from the yeast genome database. No modifications were applied. The presence of 5 stress response elements (STRE) was confirmed by sequence analysis.
Source
Promoter of the small heat-shock protein HSP12 (YFL014W) from the genome of S. Cerevisiae.