Latest revision as of 01:06, 14 December 2019

L2 spectinomycin resistance + GLE_Mut-PETase_SP20HA + cCA_MHETase_SP20HA

This composite part contains a spectinomycin resistance (BBa_K3002102), the mutant PETase with the secretion signal GLE (BBa_K3002110) and the MHETase with the cCA (BBa_K3002114), both fused with an SP20HA-tag for easy detection via HA-antibody and enhanced secretion.

The construct (LO) encodes the secretion signal GLE in front of the MUT-PETase gene and a cCA secretion signal upstream to the MHETase gene. Both, the MUT-PETase gene and the MHETase gene have a SP20-tag linked behind them. As selection marker an aadA cassette is used. Since a high yield of MHETase is visible, the cCA secretion signal must be highly functional. The MUT-PETase on the other hand is barely secreted when linked with a GLE secretion signal. This is especially noticeable, when compared to a MUT-PETase that has a cCA or ARS secretion signal upstream, as both show a way higher secretion of the MUT-PETase. The secretion of the MHETase is still higher than the secretion of the MUT-PETase, irrespective of the linked secretion signal. Both enzymes are crucial for the degradation of PET into its terephthalic acid and ethylene glycol.

Effect of the SP20 module on the secretion efficiency of MHETase and PETase. (a) Level 2 MoClo constructs harboring the aadA selection marker, and the coding sequences for MUT-PETase and MHETase equipped with the secretion signals (BBa_K3002212, BBa_K3002213, BBa_K3002214) introduced in Figure 6 and a C-terminal SP20 tag for enhancing glycosylation. See Figure 1 for the description of other parts. (b) UVM4 transformants containing the constructs shown in (a) were grown in TAP medium for seven days. Cells were centrifuged and the supernatant lyophilized, resuspended in 2xSDS buffer and analyzed by SDS-PAGE and immunoblotting with an anti-HA antibody. Transformants C12 (BBa_K3002202) and A27 (BBa_K3002200) introduced in Figures 4 and 5, respectively, served as positive controls. The black arrow points to MHETase, the white arrow to MUT-PETase.

The SP20 module increases the efficiency of protein secretion. (a) Level 2 MoClo constructs harboring the aadA selection marker, and the coding sequences for MUT-PETase and MHETase equipped with the secretion signals introduced in Figure 6. The constructs contain the coding sequence for a conventional 3xHA tag (C, K, L)(BBa_K3002202, BBa_K3002210, BBa_K3002211), or the 3xHA tag preceded by a SP20 tag to enhance glycosylation (M, N, O). See Figure 1 for the description of other parts. (b) UVM4 transformants containing the constructs C, K, L and M, N, O (BBa_K3002202, BBa_K3002210, BBa_K3002211, BBa_K3002212, BBa_K3002213, BBa_K3002214) were grown in TAP medium for seven days. Cells were centrifuged and the supernatant lyophilized, resuspended in 2xSDS buffer and analyzed by SDS-PAGE and immunoblotting with an anti-HA antibody. Transformant A27 introduced in Figures 4, served as positive control. The black arrow points to MHETase, the white arrow to MUT-PETase and the grey arrow to RPL1 (chloroplast ribosomal 50S protein L1). The RPL1 antibody was used to detect contamination from intracellular proteins.

Verification of secretion of MHETase and MUT-PETase into the medium. Transformants generated with constructs M, N, and O (BBa_K3002212, BBa_K3002213, BBa_K3002214)(Figure 8) were grown in TAP medium for seven days. Cells were centrifuged and the supernatant (s) lyophilized and resuspended in 2xSDS buffer. Cell pellets (p) were also resuspended in SDS-buffer. Both fractions were analyzed by SDS-PAGE and immunoblotting with an anti-HA antibody. The black arrow points to MHETase, the white arrow to MUT-PETase.

Quantification of secreted MHETase and MUT-PETase. (a) Transformants generated with constructs C, J, M, N, and O (BBa_K3002202, BBa_K3002208, BBa_K3002212, BBa_K3002213, BBa_K3002214)(Figure 8) were grown in TAP medium for seven days. Cells were centrifuged and the supernatant lyophilized, resuspended in 2xSDS buffer and analyzed by SDS-PAGE and immunoblotting with an anti-HA antibody. Whole-cell extracts of strain B1-TIG-HA for which concentrations of the HA-tagged TIG protein are known are loaded next to the lyophilized supernatants. The black arrow points to MHETase, the white arrows to MUT-PETase. The supernatant of a culture with the UVM4 strain were loaded as negative control. (b) Maximum cell densities, doubling times, daily growth rates, yields of MHETase and PETase and daily productivity of both combined were calculated for the transformant lines indicated.

The Chlamy Yummy Project Collection

We are proud to present our MoClo part collection for C. reinhardtii - the Chlamy Yummy project collection.

These 67 parts are all parts used during our project and were specifically designed and codon optimized for Chlamydomonas. Among them are basic parts (L0) of a novel mutant of the PETase (BBa_K3002014), the wildtype PETase and MHETase as well as a variety of functional composite parts (L1+2). Containing different tags as well as selection markers, this collection serves as a perfect base for plastic degradation projects with Chlamydomonas. These parts were tested and optimized thoroughly and we can guarantee that they work 100%. Because this is a MoClo collection, the parts are highly standardized for worldwide application. The combination with other part collections works fast and easy. While in MoClo, nomenclature is a bit different from the iGEM BioBricks, it is quickly explained:

Level 0 parts are equivalent to basic parts, e.g. Promoters, coding sequences, etc.

Level 1 parts are combinations of basic parts and usually form functional transcription units.

Level 2 parts are combinations of Level 1 parts, in case you want to transfer multiple transcription units at once. For example, you can pair your gene of interest with a selection marker.

The great thing about the Chlamy Yummy Collection and MoClo is that the ligation works in a one pot, one step reaction, as the Type IIs restriction enzymes cut out their own recognition sites. This way, multiple constructs can be combined linearly in a fixed order to create complex structures. This is ensured by the standardized overlaps that assign the parts one of 10 positions in the final constructs. After trying MoClo once, you won’t go back to traditional ligation. It is incredibly easy and reliable. Visit our parts site to get an overview over all parts.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal EcoRI site found at 2401
Illegal EcoRI site found at 5435
Illegal PstI site found at 3503
Illegal PstI site found at 4476
Illegal PstI site found at 6777
Illegal PstI site found at 7101
Illegal PstI site found at 7444
Illegal PstI site found at 8254
12
INCOMPATIBLE WITH RFC[12]
Illegal EcoRI site found at 2401
Illegal EcoRI site found at 5435
Illegal NheI site found at 2665
Illegal NheI site found at 5699
Illegal PstI site found at 3503
Illegal PstI site found at 4476
Illegal PstI site found at 6777
Illegal PstI site found at 7101
Illegal PstI site found at 7444
Illegal PstI site found at 8254
Illegal NotI site found at 7112
21
INCOMPATIBLE WITH RFC[21]
Illegal EcoRI site found at 2401
Illegal EcoRI site found at 5435
Illegal BglII site found at 8022
23
INCOMPATIBLE WITH RFC[23]
Illegal EcoRI site found at 2401
Illegal EcoRI site found at 5435
Illegal PstI site found at 3503
Illegal PstI site found at 4476
Illegal PstI site found at 6777
Illegal PstI site found at 7101
Illegal PstI site found at 7444
Illegal PstI site found at 8254
25
INCOMPATIBLE WITH RFC[25]
Illegal EcoRI site found at 2401
Illegal EcoRI site found at 5435
Illegal PstI site found at 3503
Illegal PstI site found at 4476
Illegal PstI site found at 6777
Illegal PstI site found at 7101
Illegal PstI site found at 7444
Illegal PstI site found at 8254
Illegal NgoMIV site found at 1401
Illegal NgoMIV site found at 1584
Illegal NgoMIV site found at 1694
Illegal NgoMIV site found at 3238
Illegal NgoMIV site found at 3265
Illegal NgoMIV site found at 5036
Illegal NgoMIV site found at 6710
1000
COMPATIBLE WITH RFC[1000]

@@ Line 5: / Line 5: @@
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 <p>
-The construct encodes the secretion signal GLE in front of the MUT-PETase gene and a cCA secretion signal upstream to the MHETase gene. Both, the MUT-PETase gene and the MHETase gene have a SP20-tag linked behind them. As selection marker an aadA cassette is used. Since a high yield of MHETase is visible, the cCA secretion signal must be highly functional. The MUT-PETase on the other hand is barely secreted when linked with a GLE secretion signal. This is especially noticeable, when compared to a MUT-PETase that has a cCA or ARS secretion signal upstream, as both show a way higher secretion of the MUT-PETase. The secretion of the MHETase is still higher than the secretion of the MUT-PETase, irrespective of the linked secretion signal. Both enzymes are crucial for the degradation of PET into its terephthalic acid and ethylene glycol.
+This composite part contains a spectinomycin resistance (BBa_K3002102), the mutant PETase with the secretion signal GLE (BBa_K3002110) and the MHETase with the cCA (BBa_K3002114), both fused with an SP20HA-tag for easy detection via HA-antibody and enhanced secretion.
+</p>
+<p>
+The construct (LO) encodes the secretion signal GLE in front of the MUT-PETase gene and a cCA secretion signal upstream to the MHETase gene. Both, the MUT-PETase gene and the MHETase gene have a SP20-tag linked behind them. As selection marker an aadA cassette is used. Since a high yield of MHETase is visible, the cCA secretion signal must be highly functional. The MUT-PETase on the other hand is barely secreted when linked with a GLE secretion signal. This is especially noticeable, when compared to a MUT-PETase that has a cCA or ARS secretion signal upstream, as both show a way higher secretion of the MUT-PETase. The secretion of the MHETase is still higher than the secretion of the MUT-PETase, irrespective of the linked secretion signal. Both enzymes are crucial for the degradation of PET into its terephthalic acid and ethylene glycol.
 <p/>
 <div class="figure">
 <img src="https://2019.igem.org/wiki/images/9/90/T--TU_Kaiserslautern--resultsFigure7.svg"/>
-<p class="caption"><span class="phat">Effect of the SP20 module on the secretion efficiency of MHETase.
+<p class="caption"><span class="phat">Effect of the SP20 module on the secretion efficiency of MHETase and PETase.
-</span><span class="accent">(a)</span> Level 2 MoClo constructs harboring the aadA selection marker, and the coding sequences for MUT-PETase and MHETase equipped with the secretion signals introduced in Figure 6 and a C-terminal SP20 tag for enhancing glycosylation. See Figure 1 for the description of other parts. <span class="accent">(b)</span> UVM4 transformants containing the constructs shown in <span class="accent">(a)</span> were grown in TAP medium for seven days. Cells were centrifuged and the supernatant lyophilized, resuspended in 2xSDS buffer and analyzed by SDS-PAGE and immunoblotting with an anti-HA antibody. Transformants C12 and A27 introduced in Figures 4 and 5, respectively, served as positive controls. The black arrow points to MHETase, the white arrow to MUT-PETase.
+</span><span class="accent">(a)</span> Level 2 MoClo constructs harboring the aadA selection marker, and the coding sequences for MUT-PETase and MHETase equipped with the secretion signals (<a href="https://parts.igem.org/Part:BBa_K3002212">BBa_K3002212</a>, <a href="https://parts.igem.org/Part:BBa_K3002213">BBa_K3002213</a>, <a href="https://parts.igem.org/Part:BBa_K3002214">BBa_K3002214</a>) introduced in Figure 6 and a C-terminal SP20 tag for enhancing glycosylation. See <a href="https://2019.igem.org/Team:TU_Kaiserslautern/Results">Figure 1</a> for the description of other parts. <span class="accent">(b)</span> UVM4 transformants containing the constructs shown in <span class="accent">(a)</span> were grown in TAP medium for seven days. Cells were centrifuged and the supernatant lyophilized, resuspended in 2xSDS buffer and analyzed by SDS-PAGE and immunoblotting with an anti-HA antibody. Transformants C12 (<a href="https://parts.igem.org/Part:BBa_K3002202">BBa_K3002202</a>) and A27 (<a href="https://parts.igem.org/Part:BBa_K3002200">BBa_K3002200</a>) introduced in Figures 4 and 5, respectively, served as positive controls. The black arrow points to MHETase, the white arrow to MUT-PETase.
 </p>
-</div><p>, </p><div class="figure">
+</div>
 <div class="figure">
 <img src="https://2019.igem.org/wiki/images/0/0a/T--TU_Kaiserslautern--resultsFigure8.svg"/>
 <p class="caption"><span class="phat">The SP20 module increases the efficiency of protein secretion.
-</span><span class="accent">(a)</span> Level 2 MoClo constructs harboring the aadA selection marker, and the coding sequences for MUT-PETase and MHETase equipped with the secretion signals introduced in Figure 6. The constructs contain the coding sequence for a conventional 3xHA tag (C, K, L), or the 3xHA tag preceded by a SP20 tag to enhance glycosylation (M, N, O). See Figure 1 for the description of other parts. <span class="accent">(b)</span> UVM4 transformants containing the constructs C, K, L and M, N, O  were grown in TAP medium for seven days. Cells were centrifuged and the supernatant lyophilized, resuspended in 2xSDS buffer and analyzed by SDS-PAGE and immunoblotting with an anti-HA antibody. Transformant A27 introduced in Figures 4, served as positive control. The black arrow points to MHETase, the white arrow to MUT-PETase and the grey arrow to RPL1 (chloroplast ribosomal 50S protein L1). The RPL1 antibody was used to detect contamination from intracellular proteins.
+</span><span class="accent">(a)</span> Level 2 MoClo constructs harboring the aadA selection marker, and the coding sequences for MUT-PETase and MHETase equipped with the secretion signals introduced in Figure 6. The constructs contain the coding sequence for a conventional 3xHA tag (C, K, L)(<a href="https://parts.igem.org/Part:BBa_K3002202">BBa_K3002202</a>, <a href="https://parts.igem.org/Part:BBa_K3002210">BBa_K3002210</a>, <a href="https://parts.igem.org/Part:BBa_K3002211">BBa_K3002211</a>), or the 3xHA tag preceded by a SP20 tag to enhance glycosylation (M, N, O). See Figure 1 for the description of other parts. <span class="accent">(b)</span> UVM4 transformants containing the constructs C, K, L and M, N, O  (<a href="https://parts.igem.org/Part:BBa_K3002202">BBa_K3002202</a>, <a href="https://parts.igem.org/Part:BBa_K3002210">BBa_K3002210</a>, <a href="https://parts.igem.org/Part:BBa_K3002211">BBa_K3002211</a>, <a href="https://parts.igem.org/Part:BBa_K3002212">BBa_K3002212</a>, <a href="https://parts.igem.org/Part:BBa_K3002213">BBa_K3002213</a>, <a href="https://parts.igem.org/Part:BBa_K3002214">BBa_K3002214</a>)  were grown in TAP medium for seven days. Cells were centrifuged and the supernatant lyophilized, resuspended in 2xSDS buffer and analyzed by SDS-PAGE and immunoblotting with an anti-HA antibody. Transformant A27 introduced in Figures 4, served as positive control. The black arrow points to MHETase, the white arrow to MUT-PETase and the grey arrow to RPL1 (chloroplast ribosomal 50S protein L1). The RPL1 antibody was used to detect contamination from intracellular proteins.
 </p>
-</div><p>
+</div>
-<div class="figure">
-<img src="https://2019.igem.org/wiki/images/5/58/T--TU_Kaiserslautern--resultsFigure9.svg"/>
-<p class="caption"><span class="phat">Identification of MHETase and MUT-PETase by LC-MS/MS.
-</span><span class="accent">(a)</span> Transformants generated with construct L2N <span class="accent">(d)</span> were grown in TAP medium for seven days. Cells were centrifuged and the supernatant lyophilized, resuspended in 2xSDS buffer and analyzed by SDS-PAGE and immunoblotting with an anti-HA antibody. Protein bands corresponding to those detected with the anti-HA antibody in a gel run in parallel and stained with Coomassie brilliant blue were excised, in-gel digested with trypsin and analyzed by LC-MS/MS. Peptides identified by LC-MS/MS for MHETase (green) and MUT-PETase (purple) are indicated.  <span class="accent">(b, c)</span> Sequences of MHETase and MUT-PETase with the peptides detected by LC-MS/MS are highlighted in green and purple, respectively.
-</p>
-</div><p>
 <div class="figure">
 <img src="https://2019.igem.org/wiki/images/2/24/T--TU_Kaiserslautern--resultsFigure10.svg"/>
 <p class="caption"><span class="phat">Verification of secretion of MHETase and MUT-PETase into the medium.
-</span>Transformants generated with constructs M, N, and O (Figure 8) were grown in TAP medium for seven days. Cells were centrifuged and the supernatant (s) lyophilized and resuspended in 2xSDS buffer. Cell pellets (p) were also resuspended in SDS-buffer. Both fractions were analyzed by SDS-PAGE and immunoblotting with an anti-HA antibody. The black arrow points to MHETase, the white arrow to MUT-PETase.
+</span>Transformants generated with constructs M, N, and O (<a href="https://parts.igem.org/Part:BBa_K3002212">BBa_K3002212</a>, <a href="https://parts.igem.org/Part:BBa_K3002213">BBa_K3002213</a>, <a href="https://parts.igem.org/Part:BBa_K3002214">BBa_K3002214</a>)(Figure 8) were grown in TAP medium for seven days. Cells were centrifuged and the supernatant (s) lyophilized and resuspended in 2xSDS buffer. Cell pellets (p) were also resuspended in SDS-buffer. Both fractions were analyzed by SDS-PAGE and immunoblotting with an anti-HA antibody. The black arrow points to MHETase, the white arrow to MUT-PETase.
-</p>
-</div><p>
-<div class="figure">
-<img src="https://2019.igem.org/wiki/images/8/8b/T--TU_Kaiserslautern--resFig14.png"/>
-<p class="caption"><span class="phat">Growth and secretion of MUT-PETase and MHETase in UVM4 transformant N6 under different conditions.
-</span><span class="accent">(a)</span> Growth curves of the UVM4 recipient strain and UVM4 transformant N6 (Figure 8) at 25°C, 80 µE and 33°C, 170 µE. UVM4 and transformant N6 were inoculated in 50 mL with 2*10<sup>5</sup> cells/mL. Growth was measured by counting cells for 8 days. Error bars represent the standard error of three biological replicates. <span class="accent">(b)</span> Time course of MHETase and MUT-PETase secretion into TAP medium. 2 mL of each sample was lyophilized, desalted and resuspended in 2xSDS loading buffer. 10 µl of each sample were separated via SDS-PAGE and analyzed by immunoblotting with an anti-HA antibody. An antibody against chloroplast ribosomal 50S protein L1 (RPL1) was used to detect contaminations from cellular proteins. The black arrow points to MHETase, the white arrow to MUT-PETase and the grey arrow to RPL1. <span class="accent">(c-f)</span> Bright-field images of strains UVM4 and N6 grown grown for 3 days at 25°C and 89 µE or at 33°C and 170 µE.
 </p>
 </div>
 <div class="figure">
-<img src="https://2019.igem.org/wiki/images/8/86/T--TU_Kaiserslautern--resFig15.png"/>
+<img src="https://2019.igem.org/wiki/images/e/ea/T--TU_Kaiserslautern--resultsFigure11.svg"/>
-<p class="caption"><span class="phat">Growth and secretion of MUT-PETase and MHETase in CC-4533 transformant M8 under different conditions.
+<p class="caption"><span class="phat">Quantification of secreted MHETase and MUT-PETase.
-</span><span class="accent">(a)</span> Growth curves of the CC-4533 recipient strain and CC-4533 transformant M8 (Figure 12) at 25°C, 80 µE and 33°C, 170 µE. CC-4533 and transformant M8 were inoculated in 50 mL with 2*10<sup>5</sup> cells/mL. Growth was measured by counting cells for 8 days. Error bars represent the standard error of three biological replicates. <span class="accent">(b)</span> Time course of MHETase and MUT-PETase secretion into TAP medium. 2 mL of each sample was lyophilized, desalted and resuspended in 2xSDS loading buffer. 10 µl of each sample were separated via SDS-PAGE and analyzed by immunoblotting with an anti-HA antibody. An antibody against chloroplast ribosomal 50S protein L1 (RPL1) was used to detect contaminations from cellular proteins. The black arrow points to MHETase, the white arrow to MUT-PETase and the grey arrow to RPL1. <span class="accent">(c-f)</span> Bright-field images of strains CC-4533 and M8 grown grown for 3 days at 25°C and 89 µE or at 33°C and 170 µE.
+</span><span class="accent">(a)</span> Transformants generated with constructs C, J, M, N, and O (<a href="https://parts.igem.org/Part:BBa_K3002202">BBa_K3002202</a>, <a href="https://parts.igem.org/Part:BBa_K3002208">BBa_K3002208</a>, <a href="https://parts.igem.org/Part:BBa_K3002212">BBa_K3002212</a>, <a href="https://parts.igem.org/Part:BBa_K3002213">BBa_K3002213</a>, <a href="https://parts.igem.org/Part:BBa_K3002214">BBa_K3002214</a>)(Figure 8) were grown in TAP medium for seven days. Cells were centrifuged and the supernatant lyophilized, resuspended in 2xSDS buffer and analyzed by SDS-PAGE and immunoblotting with an anti-HA antibody. Whole-cell extracts of strain B1-TIG-HA for which concentrations of the HA-tagged TIG protein are known are loaded next to the lyophilized supernatants. The black arrow points to MHETase, the white arrows to MUT-PETase. The supernatant of a culture with the UVM4 strain were loaded as negative control. <span class="accent">(b)</span> Maximum cell densities, doubling times, daily growth rates, yields of MHETase and PETase and daily productivity of both combined were calculated for the transformant lines indicated.
-</p>
-</div><p>
-<div class="figure">
-<img src="https://2019.igem.org/wiki/images/a/ad/T--TU_Kaiserslautern--resFig16.png"/>
-<p class="caption"><span class="phat">Growth and secretion of MUT-PETase and MHETase in CC-4533 transformant M8 in two photobioreactors.
-</span><span class="accent">(a, b)</span> Time course analysis of secreted MUT-PETase and MHETase in bioreactors A <span class="accent">(a)</span> and B <span class="accent">(b)</span>. The cell density in bioreactor A was held at a higher cell density than that in bioreactor B. Samples were taken once or twice a day starting at 48.2 h after inoculation. Lyophilized cell-free media was resuspended in 2xSDS loading buffer and analysed by immuno-blotting using an HA-antibody. <span class="accent">(c)</span> Cell growth in the Bioreactors A and B at 25°C.
-</p>
-</div><p>
-<div class="figure">
-<img src="https://2019.igem.org/wiki/images/b/b3/T--TU_Kaiserslautern--resultsFigure19.svg"/>
-<p class="caption"><span class="phat">Purification of HA-tagged MUT-PETase and MHETase from <i>Chlamydomonas</i> and activity measurement against BHET by reversed-phase HPLC.
-</span><span class="accent">(a)</span> Affinity purification of MUT-PETase and MHETase from <i>Chlamydomonas</i> by anti-HA magnetic beads. Transformants M5, C12 and untransformed UVM4 were inoculated in TAP for seven days. Cultures were centrifuged and supernatants incubated with anti-HA magnetic beads for 1 h. Enzymes were purified via biomagnetic separation. Samples of the unconcentrated supernatant (S), of the washing step (W), of the eluted proteins (E) and after 96 h incubation with BHET (A.I.) were analyzed by immunoblotting using an anti-HA antibody. <span class="accent">(b)</span> Proteins eluted from <i>Chlamydomonas</i> transformant M5 (producing MUT-PETase and MHETase) and the UVM4 strain (not producing MUT-PETase and MHETase) were incubated with 1 mM BHET in sodium phosphate (NaPi) buffer at 30°C for 96 h. The standard containing 1 mM TPA, MHET and BHET dissolved in DMSO is shown on top.
 </p>
 </div>
-<div class="figure">
-<img src="https://2019.igem.org/wiki/images/7/74/T--TU_Kaiserslautern--resultsFigure20.svg"/>
-<p class="caption"><span class="phat">Measurement of activity of MHETase and MUT-PETase from <i>Chlamydomonas</i> against PET by reversed-phase HPLC.
-</span>Transformant M8 and parent strain CC-4533 (CliP) were inoculated in HMP medium for seven days. M8 contains construct L2M encoding MUT-PETase and MHETase tagged with the SP20-module and secretion signal cCA (Figure 13). The cultures were centrifuged, and the supernatants concentrated 20-fold with ultracentrifuge filters and rebuffered in glycine buffer. <span class="accent">(a)</span> A standard of 1 mM TPA, MHET, and BHET dissolved in DMSO was measured by HPLC. <span class="accent">(b)</span> The 20-fold concentrated medium of transformant M8 was incubated with PET film at 25°C for 96 h and measured with HPLC. <span class="accent">(c)</span> The 20-fold concentrated medium of parent strain CC-4533 (CliP) was incubated with PET film at 25°C for 96 h and measured with HPLC. <span class="accent">(d)</span> The glycine buffer was measured with HPLC. The same measurements are displayed, but the scaling of the axis was set to 2000 mAU on the left and to 50 mAU on the right.
-</p>
-</div><p>
-</p><div class="figure">
-<img src="https://2019.igem.org/wiki/images/6/6c/T--TU_Kaiserslautern--resultsFigure21.svg"/>
-<p class="caption"><span class="phat">Activity measurement of MHETase and MUT-PETase from <i>Chlamydomonas</i> against BHET by reversed-phase HPLC.
-</span>Transformant M5 and parent strain UVM4 were inoculated in HMP medium for seven days. M5 contains construct L2M encoding MUT-PETase and MHETase tagged with the SP20-module and secretion signal cCA (Figure 8). The cultures were centrifuged, and the supernatants concentrated 20-fold with ultracentrifuge filters and rebuffered in glycine buffer. <span class="accent">(a)</span> A standard of 1 mM TPA, MHET, and BHET dissolved in DMSO was measured by HPLC. <span class="accent">(b)</span> The 20-fold concentrated medium of M5 was incubated with BHET at 30°C for 48 h and measured by HPLC. <span class="accent">(c)</span> The 20-fold concentrated medium of parent strain UVM4 was incubated with BHET and measured by HPLC. <span class="accent">(d)</span> The glycine buffer was measured with HPLC. <span class="accent">(e)</span> Peak areas of the shown measurements in mAu*s.
-</p>
-</div><p>
-</p><div class="figure">
-<img src="https://2019.igem.org/wiki/images/e/ec/T--TU_Kaiserslautern--resultsFigure22.svg"/>
-<p class="caption"><span class="phat">Activity measurement of MHETase from <i>Chlamydomonas</i> against MHET by reversed-phase HPLC.
-</span>Transformant M5 and parent strain UVM4 were inoculated in HMP medium for seven days. M5 contains construct L2M encoding MUT-PETase and MHETase tagged with the SP20-module and secretion signal cCA (Figure 8). The cultures were centrifuged, and the supernatants concentrated 20-fold with ultracentrifuge filters and rebuffered in glycine buffer. Samples were incubated with 1 mM MHET at 30°C for 48 h. <span class="accent">(a)</span> A standard of 1 mM TPA, MHET, and BHET dissolved in DMSO was measured by HPLC. <span class="accent">(b)</span> Measurement of M5 supernatant with HPLC. <span class="accent">(c)</span> Measurement of UVM4 supernatant with HPLC. <span class="accent">(d)</span> The glycine buffer was measured with HPLC. <span class="accent">(e)</span> Peak areas of the shown measurements in mAu*s.
-</p>
-</div><p>
-<div class="figure">
-<img src="https://2019.igem.org/wiki/images/2/24/T--TU_Kaiserslautern--resultsFigure23.svg"/>
-<p class="caption"><span class="phat">Measurement of activity of MUT-PETase and MHETase from <i>Chlamydomonas</i> at 25°C and 33°C by reversed-phase HPLC.
-</span>Transformant M8 and parent strain CC-4533 (CliP) were inoculated in HMP medium for seven days. M8 contains construct L2M encoding MUT-PETase and MHETase tagged with the SP20-module and secretion signal cCA (Figure 13). The cultures were centrifuged, and the supernatants concentrated 20-fold with ultracentrifuge filters and rebuffered in glycine buffer. Samples were incubated with PET film or MHET at 25°C or 33°C for 96 h. <span class="accent">(a)</span> A standard of 1 mM TPA, MHET, and BHET dissolved in DMSO was measured by HPLC. Measurement of M8 medium incubated with MHET at 25°C <span class="accent">(b)</span> and 33°C <span class="accent">(c)</span>. Measurement of M8 medium incubated with PET at 25°C (d, e) and 33°C (f, g). measured by HPLC. The measurements shown in <span class="accent">(d)</span> and <span class="accent">(e)</span>, and in <span class="accent">(f)</span> and <span class="accent">(g)</span> are the same, but the axis scaling was set to 2000 mAU in <span class="accent">(d)</span> and <span class="accent">(f)</span> and to 50 mAU in <span class="accent">(e)</span> and <span class="accent">(g)</span>. Note that the shifts in retention times are due to the clogging of the HPLC.
-</p>
-</p><div class="figure">
-<img src="https://2019.igem.org/wiki/images/c/c0/T--TU_Kaiserslautern--resultsFigure24.svg"/>
-<p class="caption"><span class="phat">Measurement of activity of MUT-PETase and MHETase from Chlamydomonas at 30°C and 40°C by reversed-phase HPLC.
-</span>Transformant M5 and parent strain UVM4 were inoculated in HMP medium for seven days. M5 contains construct L2M encoding MUT-PETase and MHETase tagged with the SP20-module and secretion signal cCA (Figure 8). The cultures were centrifuged, and the supernatants concentrated 20-fold with ultracentrifuge filters and rebuffered in glycine buffer. Samples were incubated with 1 mM BHET at 30°C (left) or 40°C (right) for 96 h. <span class="accent">(a)</span> A standard of 1 mM TPA, MHET, and BHET dissolved in DMSO was measured by HPLC. <span class="accent">(b, e)</span> Measurement of M5 medium incubated with BHET at 30°C <span class="accent">(b)</span> and 40°C <span class="accent">(e)</span>. <span class="accent">(c, f)</span> Measurement of UVM4 medium incubated with BHET at 30°C <span class="accent">(c)</span> and 40°C  <span class="accent">(f)</span>. <span class="accent">(d, g)</span> Measurement of glycine buffer incubated with BHET at 30°C <span class="accent">(d)</span> and 40°C <span class="accent">(g)</span>. <span class="accent">(h)</span> Peak areas of the shown measurements in mAu*s.
-</p>
-</div><p>
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-The great thing about the Kaiser Collection and MoClo is that the ligation works in a one pot, one step reaction, as the Type IIs restriction enzymes cut out their own recognition sites. This way, multiple constructs can be combined linearly in a fixed order to create complex structures. This is ensured by the standardized overlaps that assign the parts one of 10 positions in the final constructs.
+The great thing about the Chlamy Yummy Collection and MoClo is that the ligation works in a one pot, one step reaction, as the Type IIs restriction enzymes cut out their own recognition sites. This way, multiple constructs can be combined linearly in a fixed order to create complex structures. This is ensured by the standardized overlaps that assign the parts one of 10 positions in the final constructs.
 After trying MoClo once, you won’t go back to traditional ligation. It is incredibly easy and reliable.
 Visit our <a href="https://2019.igem.org/Team:TU_Kaiserslautern/Parts">parts site</a> to get an overview over all parts.

Difference between revisions of "Part:BBa K3002214"

Latest revision as of 01:06, 14 December 2019

The Chlamy Yummy Project Collection