Difference between revisions of "Part:BBa K3042006"
 
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DinoIII is a dinoflagellate expression system that was constructed on the basis of genomic and transcriptic data from many dinoflagellates and created by the University of Connecticut. DinoIII- RFP is a dinoflagellate optimized reporter plasmid that contains a red fluorescence protein. Designed by GSU’s iGEM team, it will be used to transform into the dinoflagellate, O.Marina. The original Dino III vector included a green fluorescence protein which for O.marina, a heterotrophic dinoflagellate, is not an issue. However, because we plan to transform Symbiodinium with this composite part, RFP was designated to replace GFP to prevent inhibition of the Symbiodinium’s photosynthetic abilities.  
 
DinoIII is a dinoflagellate expression system that was constructed on the basis of genomic and transcriptic data from many dinoflagellates and created by the University of Connecticut. DinoIII- RFP is a dinoflagellate optimized reporter plasmid that contains a red fluorescence protein. Designed by GSU’s iGEM team, it will be used to transform into the dinoflagellate, O.Marina. The original Dino III vector included a green fluorescence protein which for O.marina, a heterotrophic dinoflagellate, is not an issue. However, because we plan to transform Symbiodinium with this composite part, RFP was designated to replace GFP to prevent inhibition of the Symbiodinium’s photosynthetic abilities.  
  
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https://2019.igem.org/wiki/images/3/38/T--Georgia_State--dinoIIIrfp.png
  
 
https://2019.igem.org/wiki/images/0/08/T--Georgia_State--res3wiki.png
 
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https://2019.igem.org/wiki/images/e/ee/T--Georgia_State--res4wiki.png
 
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<img src="T--Georgia State--dinoIIIrfp.png"></img>
 
  
 
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Latest revision as of 03:35, 22 October 2019


Dino III RFP Plasmid

DinoIII is a dinoflagellate expression system that was constructed on the basis of genomic and transcriptic data from many dinoflagellates and created by the University of Connecticut. DinoIII- RFP is a dinoflagellate optimized reporter plasmid that contains a red fluorescence protein. Designed by GSU’s iGEM team, it will be used to transform into the dinoflagellate, O.Marina. The original Dino III vector included a green fluorescence protein which for O.marina, a heterotrophic dinoflagellate, is not an issue. However, because we plan to transform Symbiodinium with this composite part, RFP was designated to replace GFP to prevent inhibition of the Symbiodinium’s photosynthetic abilities.

T--Georgia_State--dinoIIIrfp.png

T--Georgia_State--res3wiki.png

T--Georgia_State--res4wiki.png


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 693
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1764
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1376
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 693
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 693
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1746