Difference between revisions of "Part:BBa K2916011"

Latest revision as of 03:26, 22 October 2019

MK protein equipped with a 6x HIS affinity tag

Adenylate kinase (Myokinase) is a protein that is used in the PURE and OnePot PURE cell-free systems.

Usage and Biology

MK participates in aminoacyl-tRNA based protein biosynthesis as a reaction catalysing enzyme.

In our part, besides the sequence encoding for the protein we also have a hexahistidine-tag to allow us purify the protein.

In our project we used MK as a part of the protein solution needed for OnePot PURE cell-free system produced with the method of gravity flow affinity chromatography, as described in the protocol we designed.

Characterization

All the data regarding the characterization of that part can be found in the composite part: BBa_K2916046 .

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal XhoI site found at 583
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 403
Illegal BsaI.rc site found at 433

@@ Line 7: / Line 7: @@
 <!-- Add more about the biology of this part here-->
 ===Usage and Biology===
-Used in OnePot PURE
+MK participates in aminoacyl-tRNA based protein biosynthesis as a reaction catalysing enzyme.
 In our part, besides the sequence encoding for the protein we also have a hexahistidine-tag to allow us purify the protein.
+In our project we used MK as a part of the protein solution needed for <html><a style="padding: 0px; margin: 0px;" href="https://2019.igem.org/Team:EPFL/OnePot_Pure"> OnePot PURE cell-free system </a></html> produced with the method of gravity flow affinity chromatography, as described in the <html><a style="padding: 0px; margin: 0px;" href="https://www.protocols.io/view/protein-purification-for-onepot-pure-cell-free-sys-8auhsew"> protocol </a></html> we designed.